SNAT2 CRISPR/Cas9 KO Plasmid (m): sc-426728
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SNAT2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the SNAT2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SNAT2 Antibody (G-8): sc-166366
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SNAT2 CRISPR/Cas9 KO Plasmid (m) | sc-426728 | 20 µg | $397.00 |
Overview
Slc38a2 encodes the mouse sodium-coupled neutral amino acid transporter SNAT2 (SLC38A2), a system A transporter that mediates uptake of small neutral amino acids such as glutamine, alanine, and serine in a Na⁺-dependent manner. SNAT2 supports cellular nutrient sensing and metabolic adaptation, linking amino acid availability to stress-responsive programs including the integrated stress response/ATF4 axis and downstream regulation of mTORC1 signaling. By shaping intracellular amino acid pools, SNAT2 influences redox balance, bioenergetics, and protein synthesis in proliferating and differentiating cells. Dysregulated amino acid transport and glutamine handling are commonly studied in contexts such as metabolic remodeling, immune cell function, and cancer biology, making Slc38a2 a useful node for probing nutrient-dependent phenotypes in mouse models.
SNAT2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Slc38a2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Slc38a2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Slc38a2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish SNAT2 protein expression.
This CRISPR knockout system enables efficient generation of Slc38a2-deficient cell models for investigation of SNAT2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Slc38a2 exon(s) critical for SNAT2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Slc38a2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by SNAT2 CRISPR/Cas9 KO Plasmid (m) and SNAT2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Slc38a2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by SNAT2 HDR Plasmid (m) and SNAT2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Slc38a2 homology arms to support homology-directed repair at defined Slc38a2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.