Shank 3 CRISPR/Cas9 KO Plasmid (m): sc-425500
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Shank 3 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Shank 3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Shank 3 Antibody (C-4): sc-377088
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Shank 3 CRISPR/Cas9 KO Plasmid (m) | sc-425500 | 20 µg | $397.00 |
Overview
Shank3 encodes Shank 3, a multidomain scaffold protein concentrated at excitatory postsynaptic densities where it organizes glutamatergic synapses by coupling receptors and signaling enzymes to the actin cytoskeleton. Through interactions with PSD-95/GKAP/Homer complexes, Shank 3 supports synaptic maturation, spine morphogenesis, and activity-dependent plasticity, integrating pathways such as mGluR signaling, CaMK/ERK cascades, and cytoskeletal remodeling. In mouse, Shank3 function is widely used to study mechanisms of synaptic homeostasis and circuit connectivity underlying neurodevelopmental phenotypes. Disruption of SHANK3-associated networks is linked to autism spectrum disorder–related biology and Phelan–McDermid syndrome, making it a key target for modeling synaptopathies in experimental systems.
Shank 3 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Shank3 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Shank3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Shank3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Shank 3 protein expression.
This CRISPR knockout system enables efficient generation of Shank3-deficient cell models for investigation of Shank 3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Shank3 exon(s) critical for Shank 3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Shank3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Shank 3 CRISPR/Cas9 KO Plasmid (m) and Shank 3 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Shank3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Shank 3 HDR Plasmid (m) and Shank 3 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Shank3 homology arms to support homology-directed repair at defined Shank3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.