SG2NA Double Nickase Plasmid (h): sc-405152-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SG2NA Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- SG2NA Double Nickase Plasmid (h) and SG2NA Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting STRN3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SG2NA Antibody (S68): sc-13562
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SG2NA Double Nickase Plasmid (h) | sc-405152-NIC | 20 µg | $410.00 | |||
SG2NA Double Nickase Plasmid (h2) | sc-405152-NIC-2 | 20 µg | $410.00 |
STRN3 encodes striatin-3 (SG2NA), a member of the striatin family of WD-repeat scaffold proteins that organize multiprotein signaling assemblies. SG2NA participates in STRIPAK-related complexes and supports spatial regulation of serine/threonine phosphatases and kinases, linking membrane-associated signaling to cytoskeletal remodeling and vesicular trafficking. Through these interactions, STRN3 can influence pathways controlling cell polarity, migration, and cell-cycle progression, and its dysregulation has been investigated in contexts where aberrant signaling network architecture contributes to tumorigenic phenotypes. STRN3 is also studied for roles in modulating stress-responsive signaling and protein complex dynamics across subcellular compartments.
SG2NA Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the STRN3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within STRN3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt STRN3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of STRN3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.