SEMA6D Double Nickase Plasmid (m): sc-431980-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SEMA6D Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- SEMA6D Double Nickase Plasmid (m) and SEMA6D Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Sema6d. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SEMA6D Antibody (A-8): sc-393258
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SEMA6D Double Nickase Plasmid (m) | sc-431980-NIC | 20 µg | $410.00 | |||
SEMA6D Double Nickase Plasmid (m2) | sc-431980-NIC-2 | 20 µg | $410.00 |
Sema6d encodes the mouse semaphorin SEMA6D, a transmembrane guidance cue that signals through plexin receptors to regulate contact-dependent cell–cell communication. SEMA6D modulates cytoskeletal remodeling, directional migration, and axon pathfinding, linking semaphorin–plexin signaling to Rho-family GTPase activity and downstream actin dynamics. In addition to neural patterning, SEMA6D contributes to tissue morphogenesis and vascular and immune-related processes where guidance pathways shape cell positioning. Dysregulated semaphorin signaling, including altered Sema6d expression, is used to interrogate mechanisms of aberrant innervation, inflammatory cell trafficking, and microenvironmental remodeling in disease-relevant models.
SEMA6D Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Sema6d locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Sema6d. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Sema6d function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Sema6d-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.