Sc1 CRISPR/Cas9 KO Plasmid (m): sc-420099
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Sc1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Sc1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SPARCL1 Antibody (G-5): sc-514275
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Sc1 CRISPR/Cas9 KO Plasmid (m) | sc-420099 | 20 µg | $397.00 |
Overview
Mouse Sparcl1 encodes Sc1, a secreted matricellular glycoprotein of the SPARC family that modulates extracellular matrix organization and cell–matrix adhesion. Sc1 contributes to regulation of cell migration, proliferation, and differentiation by influencing integrin signaling, focal adhesion dynamics, and tissue remodeling programs. In vascular and stromal contexts, SPARCL1 is linked to angiogenic regulation and endothelial–smooth muscle interactions, with expression changes reported in fibrosis, inflammation-associated remodeling, and tumor microenvironment biology. These properties make Sparcl1 a useful target for studying ECM-dependent signaling networks that shape tissue architecture and disease-relevant stromal phenotypes.
Sc1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Sparcl1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Sparcl1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Sparcl1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Sc1 protein expression.
This CRISPR knockout system enables efficient generation of Sparcl1-deficient cell models for investigation of Sc1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Sparcl1 exon(s) critical for Sc1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Sparcl1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Sc1 CRISPR/Cas9 KO Plasmid (m) and Sc1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Sparcl1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Sc1 HDR Plasmid (m) and Sc1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Sparcl1 homology arms to support homology-directed repair at defined Sparcl1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.