Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
RUNX1 Double Nickase Plasmid (h): sc-400803-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RUNX1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- RUNX1 Double Nickase Plasmid (h) and RUNX1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting RUNX1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: RUNX1 Antibody (A-2): sc-365644
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RUNX1 Double Nickase Plasmid (h) | sc-400803-NIC | 20 µg | $410.00 | |||
RUNX1 Double Nickase Plasmid (h2) | sc-400803-NIC-2 | 20 µg | $410.00 |
RUNX1 encodes a runt-related transcription factor that forms a DNA-binding complex with CBFB to control lineage-specific gene expression programs during definitive hematopoiesis. It regulates differentiation, self-renewal, and cell cycle coordination in hematopoietic stem and progenitor cells by integrating signals from pathways such as TGF-β/BMP, Wnt, and cytokine-driven transcriptional networks. RUNX1 also participates in chromatin remodeling and enhancer selection to establish myeloid and lymphoid transcriptional states. Dysregulation of RUNX1 through mutation, translocation, or altered expression is strongly associated with hematologic malignancy biology and impaired blood cell development, making it a central target for mechanistic studies in gene regulation and leukemogenesis models.
RUNX1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the RUNX1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within RUNX1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt RUNX1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of RUNX1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.