RNF123 Double Nickase Plasmid (m): sc-429979-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RNF123 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- RNF123 Double Nickase Plasmid (m) and RNF123 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Rnf123. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RNF123 Double Nickase Plasmid (m) | sc-429979-NIC | 20 µg | $410.00 | |||
RNF123 Double Nickase Plasmid (m2) | sc-429979-NIC-2 | 20 µg | $410.00 |
Rnf123 encodes the E3 ubiquitin ligase RNF123 (also known as KPC1), a RING finger protein that contributes to ubiquitin-dependent proteostasis by catalyzing substrate ubiquitination and directing proteins to the 26S proteasome. RNF123 has been linked to regulation of cell-cycle progression, including turnover of cell-cycle regulators such as CDKN1B/p27, and can influence checkpoints, proliferation, and cellular stress responses through ubiquitin–proteasome pathways. In mouse systems, perturbation of RNF123 function is therefore relevant for dissecting ubiquitin signaling networks that intersect with growth control, differentiation, and tissue homeostasis. Altered ubiquitination and proteasome-mediated degradation are broadly implicated in oncology and neurobiology research, making RNF123 a useful node for mechanistic studies of disease-associated proteostasis and signaling imbalance.
RNF123 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Rnf123 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Rnf123. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Rnf123 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Rnf123-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.