Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
RMP Double Nickase Plasmid (h): sc-406916-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RMP Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- RMP Double Nickase Plasmid (h) and RMP Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting URI1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: RMP Antibody (D-2): sc-376011
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RMP Double Nickase Plasmid (h) | sc-406916-NIC | 20 µg | $410.00 | |||
RMP Double Nickase Plasmid (h2) | sc-406916-NIC-2 | 20 µg | $410.00 |
Human URI1 encodes unconventional prefoldin RPB5 interactor 1 (RMP), a multifunctional factor that associates with RNA polymerase II via RPB5 and contributes to transcriptional control, proteostasis, and cellular stress responses. RMP participates in regulatory networks that influence cell growth and survival pathways, including modulation of nuclear receptor signaling and responses to genotoxic or metabolic stress. Altered URI1/RMP activity has been reported in contexts of dysregulated proliferation and oncogenic signaling, making it a useful node for studying transcription–metabolism coupling. Experimental interrogation of URI1 supports research into pathway rewiring, protein complex dynamics, and mechanisms that sustain aberrant cellular states.
RMP Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the URI1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within URI1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt URI1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of URI1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.