Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
RIP140 CRISPR/Cas9 KO Plasmid (m): sc-435290
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RIP140 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the RIP140 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RIP140 CRISPR/Cas9 KO Plasmid (m) | sc-435290 | 20 µg | $397.00 |
Overview
Mouse Nrip1 encodes RIP140 (NRIP1), a ligand-dependent nuclear receptor coregulator that predominantly acts as a transcriptional corepressor by recruiting chromatin-modifying complexes such as HDAC-containing assemblies. RIP140 integrates signaling from nuclear receptors including PPARs, ER, TR, and RXR to modulate gene programs controlling mitochondrial function, oxidative metabolism, adipocyte differentiation, and inflammatory transcriptional outputs. Through these pathways, Nrip1 is widely used to study metabolic homeostasis, energy expenditure, and macrophage-mediated inflammatory responses. Dysregulated RIP140-dependent transcriptional control has been associated with phenotypes relevant to obesity, insulin resistance, fatty liver biology, and hormone-responsive tumor models in preclinical research settings.
RIP140 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Nrip1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Nrip1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Nrip1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish RIP140 protein expression.
This CRISPR knockout system enables efficient generation of Nrip1-deficient cell models for investigation of RIP140 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Nrip1 exon(s) critical for RIP140 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Nrip1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by RIP140 CRISPR/Cas9 KO Plasmid (m) and RIP140 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Nrip1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by RIP140 HDR Plasmid (m) and RIP140 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Nrip1 homology arms to support homology-directed repair at defined Nrip1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.