Ribosomal Protein L26 CRISPR/Cas9 KO Plasmid (h): sc-406835
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Ribosomal Protein L26 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Ribosomal Protein L26 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Ribosomal Protein L26 CRISPR/Cas9 KO Plasmid (h) | sc-406835 | 20 µg | $397.00 |
Overview
RPL26 encodes ribosomal protein L26, a structural component of the 60S large ribosomal subunit that contributes to ribosome assembly and efficient translation of cytosolic mRNAs. Beyond its core role in protein synthesis, RPL26 has been linked to regulation of stress-responsive translation programs, including modulation of p53 pathway signaling through effects on translation of key transcripts. Perturbation of ribosomal protein homeostasis can trigger ribosomal stress responses and alter cell-cycle control, supporting investigation of RPL26 in contexts such as genome stability, apoptosis, and altered translational control in cancer and other proliferative disorders.
Ribosomal Protein L26 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the RPL26 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the RPL26 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the RPL26 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Ribosomal Protein L26 protein expression.
This CRISPR knockout system enables efficient generation of RPL26-deficient cell models for investigation of Ribosomal Protein L26 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting RPL26 exon(s) critical for Ribosomal Protein L26 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple RPL26 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Ribosomal Protein L26 CRISPR/Cas9 KO Plasmid (h) and Ribosomal Protein L26 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the RPL26 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Ribosomal Protein L26 HDR Plasmid (h) and Ribosomal Protein L26 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by RPL26 homology arms to support homology-directed repair at defined RPL26 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.