RFX2 CRISPR/Cas9 KO Plasmid (h): sc-406040
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- RFX2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the RFX2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RFX2 CRISPR/Cas9 KO Plasmid (h) | sc-406040 | 20 µg | $397.00 |
Overview
RFX2 (regulatory factor X2) is a winged-helix transcription factor that binds X-box motifs and coordinates gene expression programs required for ciliogenesis and motile cilia function. In human cells, RFX2 regulates networks controlling basal body docking, axonemal assembly, and intraflagellar transport, linking it to cell cycle-cilia coupling and epithelial differentiation. Dysregulation of RFX family transcriptional circuits has been associated with cilia-related developmental defects and reproductive phenotypes consistent with impaired motile cilia and flagellar biogenesis. As a transcriptional regulator upstream of multiple ciliary structural and transport genes, RFX2 is frequently studied in pathways governing mucociliary clearance, left-right patterning, and spermatogenesis-relevant gene expression.
RFX2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the RFX2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the RFX2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the RFX2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish RFX2 protein expression.
This CRISPR knockout system enables efficient generation of RFX2-deficient cell models for investigation of RFX2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting RFX2 exon(s) critical for RFX2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple RFX2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by RFX2 CRISPR/Cas9 KO Plasmid (h) and RFX2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the RFX2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by RFX2 HDR Plasmid (h) and RFX2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by RFX2 homology arms to support homology-directed repair at defined RFX2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.