Anticorpo RFC1 (B-5) raccomandato per il rilevamento di RFC1 di origine human in WB, IP, IF e ELISA

Anticorpo RFC1 (B-5): sc-271656

Anticorpo RFC1 (B-5) valutazione 5.0 di 5 di 3.
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Schede tecniche

    • Anti-RFC1 Antibody B-5 è un monoclonale di topo IgG2a κ RFC1 antibody, citato in 2 pubblicazioni, fornito in 200 µg/ml
    • generato contro gli amminoacidi 848-1147 mappati al C-terminus di RFC1 di origine human
    • RFC1 Antibody (B-5) é raccomandato per il rilevamento di RFC1 di origine human in WB, IP, IF e ELISA
    • RFC1 Antibody (B-5) é disponibile coniugato con agarose per IP; HRP per WB, IHC(P) ed ELISA; sia con phycoerythrin o FITC per IF, IHC(P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 488, Alexa Fluor® 546; Alexa Fluor® 594 o Alexa Fluor® 647 per IF, IHC (P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 680 o Alexa Fluor® 790 per WB (NIR), IF e FCM
    • Contattaci per ricevere un FREE 10 µg sample di RFC1 (B-5): sc-271656.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for RFC1 Antibody (B-5) for WB applications. These reagents are now offered in bundles with RFC1 Antibody (B-5) (see ordering information below).
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What is the recommended fixation method for immunofluorescence staining with RFC1 (B-5): sc-271656 monoclonal antibody?

Richiesta da: DefinitelyNotMatt
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Risposta da: Technical Support
Data di pubblicazione: 2017-02-25
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Rated 5 di 5 di da Nice signal in WB upon nuclear fractionation Nice signal in WB upon nuclear fractionation. Signal reduced upon transfection with shRNAs against the target protein
Data di pubblicazione: 2018-01-25
Rated 5 di 5 di da Produced superb immunofluorescence nuclear Produced superb immunofluorescence nuclear staining in methanol-fixed HeLa cells. -SCBT QC
Data di pubblicazione: 2015-02-23
Rated 5 di 5 di da Nice Western Blot data of RFC1 expression Nice Western Blot data of RFC1 expression in HeLa nuclear extract. -SCBT QC
Data di pubblicazione: 2013-10-27
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