REEP2 CRISPR/Cas9 KO Plasmid (m): sc-432536
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- REEP2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the REEP2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
REEP2 CRISPR/Cas9 KO Plasmid (m) | sc-432536 | 20 µg | $397.00 |
Overview
Reep2 encodes receptor expression-enhancing protein 2 (REEP2), a member of the REEP family implicated in shaping endoplasmic reticulum membranes and supporting ER–microtubule interactions that influence intracellular trafficking. REEP2 contributes to membrane protein handling and neurite-associated ER organization, processes important for maintaining neuronal morphology and proteostasis. Disruption of REEP family functions has been linked to axonal vulnerability and neurodegenerative mechanisms, making Reep2 a relevant target for studying pathways that impact axon maintenance and stress responses. In mouse systems, Reep2 perturbation can be used to interrogate ER remodeling, organelle dynamics, and trafficking-dependent signaling in neurons and other secretory cell types.
REEP2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Reep2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Reep2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Reep2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish REEP2 protein expression.
This CRISPR knockout system enables efficient generation of Reep2-deficient cell models for investigation of REEP2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Reep2 exon(s) critical for REEP2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Reep2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by REEP2 CRISPR/Cas9 KO Plasmid (m) and REEP2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Reep2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by REEP2 HDR Plasmid (m) and REEP2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Reep2 homology arms to support homology-directed repair at defined Reep2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.