Anticorpo Rad51 (G-9) raccomandato per il rilevamento di Rad51 di origine mouse, rat e human in WB, IP, IF, IHC(P) e ELISA

Anticorpo Rad51 (G-9): sc-377467

Anticorpo Rad51 (G-9) valutazione 4.0 di 5 di 12.
  • y_2021, m_9, d_19, h_20
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Schede tecniche

    • Rad51 Antibody G-9 è un monoclonale di topo IgG1 κ, citato in 6 pubblicazioni, fornito in 200 µg/ml
    • generato contro gli amminoacidi 1-92 di Rad51 di origine human
    • Rad51 Antibody (G-9) é raccomandato per il rilevamento di Rad51 di origine mouse, rat e human in WB, IP, IF, IHC(P) e ELISA
    • Rad51 Antibody (G-9) é disponibile coniugato con agarose per IP; HRP per WB, IHC(P) ed ELISA; sia con phycoerythrin o FITC per IF, IHC(P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 488, Alexa Fluor® 546; Alexa Fluor® 594 o Alexa Fluor® 647 per IF, IHC (P) e FCM
    • disponibile anche coniugato con Alexa Fluor® 680 o Alexa Fluor® 790 per WB (NIR), IF e FCM
    • Contattaci per ricevere un FREE 10 µg sample di Rad51 (G-9): sc-377467.
    • m-IgGκ BP-HRP is the preferred secondary detection reagent for Rad51 Antibody (G-9) for WB and IHC(P) applications. This reagent is now offered in a bundle with Rad51 Antibody (G-9) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Richiesta da: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Risposta da: Technical Support 15
Data di pubblicazione: 2017-02-07
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Rated 4 di 5 di da It works well in WB! We used it at 1:100 diluition in BSA and it works very well.
Data di pubblicazione: 2020-10-13
Rated 5 di 5 di da Great signal We Used for WB at 1:100 dilution, with human infected B lymphocytes and in Panc1 cells and had a great signal
Data di pubblicazione: 2020-02-10
Rated 1 di 5 di da not recomended for WB no signal for WB (dilution range 1:50 - 1:1000 in BSA/TBS-T or MMP/TBS-T) and only a very weak signal in IF (dilution 1:50) if the cells were fixed in 2% formaldehyde. Even then the background signal is too high to obtain a good signal.
Data di pubblicazione: 2020-01-09
Rated 4 di 5 di da Good antibody for WB Used 1:500 5% milk TBST overnight at 4 degrees. Worked very nicely.
Data di pubblicazione: 2018-03-05
Rated 1 di 5 di da Not useful for IF Tried to detect rad51 foci after DNA damage in cell line. It does not work, very few signal, nucleus barely stained (dilution 1/500)
Data di pubblicazione: 2018-02-07
Rated 5 di 5 di da Great antibody! Used for WB at 1:1000 dilution, i worked with rat cell line. See clean and strong bands between 35 and 55 kDa markers.
Data di pubblicazione: 2017-11-16
Rated 5 di 5 di da Great! Worked well for my rat cell line. Saw a clear band between 35 and 55kDa marker.
Data di pubblicazione: 2017-11-15
Rated 5 di 5 di da Excellent choice! This mouse monoclonal antibody, clone (G-9), has been validated for detection of Rad51 of mouse, rat and human origin in Western blot and IHC(P) research applications. An excellent choice!
Data di pubblicazione: 2017-01-24
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