Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PSPH CRISPR/Cas9 KO Plasmid (h): sc-403897
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PSPH CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PSPH genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PSPH Antibody (H-10): sc-271421
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PSPH CRISPR/Cas9 KO Plasmid (h) | sc-403897 | 20 µg | $397.00 |
Overview
Phosphoserine phosphatase (PSPH) catalyzes the final step of the phosphorylated serine biosynthesis pathway, converting O-phospho-L-serine to L-serine and inorganic phosphate. By controlling intracellular serine availability, PSPH supports one-carbon metabolism and nucleotide synthesis, redox balance through glutathione production, and lipid synthesis, linking amino acid metabolism to proliferative and stress-response programs. PSPH activity interfaces with metabolic rewiring observed in rapidly dividing cells and is frequently examined alongside PHGDH and PSAT1 within the serine–glycine axis. Dysregulated expression of PSPH has been associated with altered metabolic phenotypes in cancer and other disorders characterized by perturbed amino acid and one-carbon metabolism, making it relevant for mechanistic studies of metabolic vulnerability.
PSPH CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PSPH gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PSPH together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PSPH open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PSPH protein expression.
This CRISPR knockout system enables efficient generation of PSPH-deficient cell models for investigation of PSPH signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PSPH exon(s) critical for PSPH function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PSPH genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PSPH CRISPR/Cas9 KO Plasmid (h) and PSPH CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PSPH locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PSPH HDR Plasmid (h) and PSPH HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PSPH homology arms to support homology-directed repair at defined PSPH target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.