PSGR Double Nickase Plasmid (h): sc-405966-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PSGR Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PSGR Double Nickase Plasmid (h) and PSGR Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting OR51E2. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PSGR Double Nickase Plasmid (h) | sc-405966-NIC | 20 µg | $410.00 | |||
PSGR Double Nickase Plasmid (h2) | sc-405966-NIC-2 | 20 µg | $410.00 |
OR51E2 encodes the human prostate-specific G protein-coupled receptor (PSGR), an olfactory receptor ectopically expressed in prostate epithelium and other peripheral tissues. As a seven-transmembrane GPCR, PSGR is linked to ligand-dependent signal transduction that can engage cAMP/PKA signaling, calcium flux, and downstream MAPK and PI3K-associated pathways that shape epithelial differentiation, migration, and inflammatory responses. Altered OR51E2 expression has been reported in prostate cancer and related prostate pathophysiology, supporting its use as a molecular handle to interrogate tumor-associated signaling programs. OR51E2/PSGR functional studies also inform how chemosensory GPCRs regulate cellular metabolism and microenvironmental interactions outside the olfactory system.
PSGR Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the OR51E2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within OR51E2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt OR51E2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of OR51E2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.