PSGL-1 CRISPR/Cas9 KO Plasmid (m): sc-422874
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PSGL-1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PSGL-1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PSGL-1 Antibody (HECA-452): sc-53514
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PSGL-1 CRISPR/Cas9 KO Plasmid (m) | sc-422874 | 20 µg | $397.00 |
Overview
Selplg encodes P-selectin glycoprotein ligand-1 (PSGL-1), a mucin-like sialomucin expressed on leukocytes that binds selectins to mediate tethering, rolling, and firm adhesion on activated endothelium and platelets. Through regulated glycosylation and tyrosine sulfation, PSGL-1 integrates with adhesion and chemokine-driven signaling to coordinate leukocyte trafficking, extravasation, and immune synapse formation. PSGL-1 participates in inflammatory cascades by shaping neutrophil recruitment, monocyte-endothelial interactions, and platelet–leukocyte aggregates, linking vascular inflammation with immune activation. Dysregulated SELPLG/PSGL-1 function has been associated with altered host defense and inflammatory pathology, making it a useful target for mechanistic studies of leukocyte migration and immunothrombosis-related processes.
PSGL-1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Selplg gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Selplg together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Selplg open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PSGL-1 protein expression.
This CRISPR knockout system enables efficient generation of Selplg-deficient cell models for investigation of PSGL-1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Selplg exon(s) critical for PSGL-1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Selplg genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PSGL-1 CRISPR/Cas9 KO Plasmid (m) and PSGL-1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Selplg locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PSGL-1 HDR Plasmid (m) and PSGL-1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Selplg homology arms to support homology-directed repair at defined Selplg target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.