PRRG1 CRISPR/Cas9 KO Plasmid (m): sc-436837
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PRRG1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PRRG1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PRRG1 CRISPR/Cas9 KO Plasmid (m) | sc-436837 | 20 µg | $397.00 |
Overview
Mouse Prrg1 encodes PRRG1, a proline-rich transmembrane protein with a γ-carboxyglutamate (Gla) domain that suggests roles in calcium-dependent protein interactions at the cell surface. PRRG1 is implicated in adhesion-associated signaling and membrane-proximal regulatory processes that can influence cytoskeletal organization, receptor trafficking, and communication between the extracellular matrix and intracellular pathways. Expression patterns and reported associations link PRRG1 to cellular differentiation programs and tissue-specific homeostasis, making it relevant for studies of signaling perturbations in developmental and disease-related contexts. Dissecting PRRG1 function can help clarify how Gla-domain proteins modulate local signaling microenvironments and downstream transcriptional responses.
PRRG1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Prrg1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Prrg1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Prrg1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PRRG1 protein expression.
This CRISPR knockout system enables efficient generation of Prrg1-deficient cell models for investigation of PRRG1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Prrg1 exon(s) critical for PRRG1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Prrg1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PRRG1 CRISPR/Cas9 KO Plasmid (m) and PRRG1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Prrg1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PRRG1 HDR Plasmid (m) and PRRG1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Prrg1 homology arms to support homology-directed repair at defined Prrg1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.