Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PrP Double Nickase Plasmid (h): sc-401061-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PrP Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PrP Double Nickase Plasmid (h) and PrP Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PRNP. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PrP Antibody (G-12): sc-398451
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PrP Double Nickase Plasmid (h) | sc-401061-NIC | 20 µg | $410.00 | |||
PrP Double Nickase Plasmid (h2) | sc-401061-NIC-2 | 20 µg | $410.00 |
Human PRNP encodes the prion protein PrP, a glycosylphosphatidylinositol-anchored cell-surface protein enriched in the nervous system and implicated in synaptic function, copper binding, and cellular stress responses. PrP undergoes constitutive endocytic trafficking and processing through secretory and endolysosomal pathways, intersecting with proteostasis networks including the ubiquitin–proteasome system and autophagy. Misfolding and conformational conversion of PrP are central to prion biology and are associated with neurodegenerative phenotypes characterized by protein aggregation and neuronal dysfunction. PRNP is therefore widely studied in models of protein misfolding, membrane microdomain signaling, and host factors that influence susceptibility to prion-like propagation.
PrP Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PRNP locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PRNP. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PRNP function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PRNP-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.