PP2Cκ Antibody (E-8): sc-514925

PP2Cκ Antibody (E-8) is a mouse monoclonal IgG1 antibody that detects PP2Cκ in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PP2Cκ, also known as Protein phosphatase 2C isoform kappa, is a crucial 372 amino acid protein located in the mitochondrial matrix, where PP2Cκ plays a vital role in regulating mitochondrial membrane permeability transition pores. This regulation is essential for maintaining cell survival, cardiac function, and proper embryonic development. PP2Cκ′s importance is underscored by knockdown studies leading to cell death due to the loss of mitochondrial membrane potential, highlighting PP2Cκ′s protective role in cellular health. Additionally, PP2Cκ interacts specifically with the branched-chain-alpha-ketoacid dehydrogenase (BCKD) complex, facilitating the dephosphorylation of Ser293, which ultimately inhibits branched-chain amino acid metabolism. PP2Cκ shows highest expression levels in the brain, diaphragm, and heart, and exists in three isoforms produced by alternative splicing, further emphasizing PP2Cκ′s functional diversity and significance in various biological processes.

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PP2Cκ Antibody (E-8) References:

1. Regulation of PP2Cm expression by miRNA-204/211 and miRNA-22 in mouse and human cells.  |  Pan, BF., et al. 2015. Acta Pharmacol Sin. 36: 1480-6. PMID: 26592513
2. Effect of the interaction between diet composition and the PPM1K genetic variant on insulin resistance and β cell function markers during weight loss: results from the Nutrient Gene Interactions in Human Obesity: implications for dietary guidelines (NUGENOB) randomized trial.  |  Goni, L., et al. 2017. Am J Clin Nutr. 106: 902-908. PMID: 28768654
3. Diabetes and branched-chain amino acids: What is the link?  |  Bloomgarden, Z. 2018. J Diabetes. 10: 350-352. PMID: 29369529
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6. The expression of apoptosis related genes in HK-2 cells overexpressing PPM1K was determined by RNA-seq analysis.  |  Zhang, L., et al. 2022. Front Genet. 13: 1004610. PMID: 36386814
7. Comprehensive Analysis of PPMs in Pancreatic Adenocarcinoma Indicates the Value of PPM1K in the Tumor Microenvironment.  |  Zhuang, Y., et al. 2023. Cancers (Basel). 15: PMID: 36672423
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9. Branched-chain keto acids inhibit mitochondrial pyruvate carrier and suppress gluconeogenesis in hepatocytes.  |  Nishi, K., et al. 2023. Cell Rep. 42: 112641. PMID: 37310861
10. miR-743b-3p promotes hepatic lipogenesis via branched-chain amino acids (BCAA) metabolism by targeting PPM1K in aged mice.  |  Lu, T., et al. 2024. Arch Gerontol Geriatr. 123: 105424. PMID: 38565071