Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PP2A-Aβ CRISPR/Cas9 KO Plasmid (m): sc-428621
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PP2A-Aβ CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PP2A-Aβ genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PP2A-Aβ CRISPR/Cas9 KO Plasmid (m) | sc-428621 | 20 µg | $397.00 |
Overview
Ppp2r1b encodes the PP2A-Aβ scaffolding subunit of protein phosphatase 2A, a core serine/threonine phosphatase complex that organizes catalytic and regulatory subunits to control substrate specificity and localization. PP2A signaling shapes phosphorylation-dependent processes including cell-cycle progression, DNA damage responses, cytoskeletal dynamics, and MAPK/AKT pathway modulation. By tuning dephosphorylation events across multiple networks, PP2A-Aβ influences cellular homeostasis and stress adaptation in diverse tissues. Dysregulation of PP2A subunits, including scaffold components, has been linked to altered proliferative signaling and genome stability, supporting its relevance in mechanistic studies of disease-associated signaling states.
PP2A-Aβ CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Ppp2r1b gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Ppp2r1b together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Ppp2r1b open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PP2A-Aβ protein expression.
This CRISPR knockout system enables efficient generation of Ppp2r1b-deficient cell models for investigation of PP2A-Aβ signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Ppp2r1b exon(s) critical for PP2A-Aβ function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Ppp2r1b genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PP2A-Aβ CRISPR/Cas9 KO Plasmid (m) and PP2A-Aβ CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Ppp2r1b locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PP2A-Aβ HDR Plasmid (m) and PP2A-Aβ HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Ppp2r1b homology arms to support homology-directed repair at defined Ppp2r1b target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.