Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PMS2 Double Nickase Plasmid (h): sc-401600-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PMS2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PMS2 Double Nickase Plasmid (h) and PMS2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PMS2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PMS2 Antibody (B-3): sc-25315
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PMS2 Double Nickase Plasmid (h) | sc-401600-NIC | 20 µg | $410.00 | |||
PMS2 Double Nickase Plasmid (h2) | sc-401600-NIC-2 | 20 µg | $410.00 |
PMS2 encodes a core component of the DNA mismatch repair (MMR) machinery, forming the MutLα heterodimer with MLH1 to coordinate recognition and processing of base–base mismatches and insertion–deletion loops that arise during replication. Through ATPase-driven conformational switching and endonuclease activity, PMS2 helps couple mismatch detection to excision, resynthesis, and ligation, thereby maintaining genome stability and limiting mutagenesis. PMS2 function interfaces with replication fidelity, checkpoint signaling, and cellular responses to DNA damage. Disruption or dysregulation of PMS2 is linked to elevated microsatellite instability and hypermutation phenotypes that are relevant to studies of hereditary cancer predisposition and DNA repair–associated disease mechanisms.
PMS2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PMS2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PMS2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PMS2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PMS2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.