PMEPA1 Antibody (2A12): sc-293372

PMEPA1 Antibody (2A12) is a mouse monoclonal IgG2a kappa light chain antibody that detects PMEPA1 protein of human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). PMEPA1 (2A12) antibody is available as a non-conjugated anti-PMEPA1 monoclonal isotype antibody. PMEPA1, also known as prostate transmembrane protein, androgen induced 1, STAG1, or TMEPAI, is a 287 amino acid single-pass membrane protein characterized by WW-binding motifs, which are crucial for protein-protein interactions. PMEPA1 is predominantly localized to the cell membrane and highly expressed in the prostate and ovary, where PMEPA1 plays a significant role in regulating androgen receptor levels, particularly in prostate cells. PMEPA1 interaction with NEDD4 is vital for function, as PMEPA1 may facilitate ubiquitination and subsequent degradation of the androgen receptor, thereby modulating androgen signaling pathways. Notably, downregulation of PMEPA1 has been observed in prostate tumors, indicating potential role as a tumor suppressor. PMEPA1 exists in multiple alternatively spliced isoforms, which adds complexity to functional roles in cellular processes. The gene encoding PMEPA1 is located on human chromosome 20, a chromosome that comprises approximately 2% of the human genome and contains over 600 genes, some of which are implicated in various diseases, including Creutzfeldt-Jakob disease and amyotrophic lateral sclerosis.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

PMEPA1 Antibody (2A12) References:

1. A novel androgen-regulated gene, PMEPA1, located on chromosome 20q13 exhibits high level expression in prostate.  |  Xu, LL., et al. 2000. Genomics. 66: 257-63. PMID: 10873380
2. Identification of multiple proteins expressed in murine embryos as binding partners for the WW domains of the ubiquitin-protein ligase Nedd4.  |  Jolliffe, CN., et al. 2000. Biochem J. 351 Pt 3: 557-65. PMID: 11042109
3. Characterization of a novel gene, STAG1/PMEPA1, upregulated in renal cell carcinoma and other solid tumors.  |  Rae, FK., et al. 2001. Mol Carcinog. 32: 44-53. PMID: 11568975
4. PMEPA1, a transforming growth factor-beta-induced marker of terminal colonocyte differentiation whose expression is maintained in primary and metastatic colon cancer.  |  Brunschwig, EB., et al. 2003. Cancer Res. 63: 1568-75. PMID: 12670906
5. PMEPA1, an androgen-regulated NEDD4-binding protein, exhibits cell growth inhibitory function and decreased expression during prostate cancer progression.  |  Xu, LL., et al. 2003. Cancer Res. 63: 4299-304. PMID: 12907594
6. EGF- and cell-cycle-regulated STAG1/PMEPA1/ERG1.2 belongs to a conserved gene family and is overexpressed and amplified in breast and ovarian cancer.  |  Giannini, G., et al. 2003. Mol Carcinog. 38: 188-200. PMID: 14639658
7. A role for DNA methylation in regulating the growth suppressor PMEPA1 gene in prostate cancer.  |  Richter, E., et al. 2007. Epigenetics. 2: 100-9. PMID: 18174752
8. A feedback loop between the androgen receptor and a NEDD4-binding protein, PMEPA1, in prostate cancer cells.  |  Li, H., et al. 2008. J Biol Chem. 283: 28988-95. PMID: 18703514

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PMEPA1 Antibody (2A12) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-PMEPA1 Antibody (2A12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PMEPA1 Antibody (2A12) Specifications

Alternate Product Name: PMEPA1

Product Clone Name: 2A12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 181-280

Epitope Target: PMEPA1

Epitope Species: human

Product Reactivity/Detection: PMEPA1

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for PMEPA1 Antibody (2A12): N/A