Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
plexin-B1 CRISPR/Cas9 KO Plasmid (h): sc-401840
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- plexin-B1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the plexin-B1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: plexin-B1 Antibody (A-8): sc-28372
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
plexin-B1 CRISPR/Cas9 KO Plasmid (h) | sc-401840 | 20 µg | $397.00 |
Overview
PLXNB1 encodes plexin-B1, a single-pass transmembrane receptor for class 4 semaphorins that coordinates cytoskeletal remodeling and directional cell movement. Through coupling to small GTPase signaling modules, including Rho family pathways and Ras-related inputs, plexin-B1 regulates actin dynamics, adhesion turnover, and neurite guidance. Plexin-B1 activity intersects with receptor tyrosine kinase and integrin-associated signaling to influence migration and invasive behavior across multiple cell contexts. Dysregulated PLXNB1 expression or signaling has been implicated in neurodevelopmental processes and in cancer-associated phenotypes such as altered motility and metastatic potential, supporting its use as a mechanistic node in pathway studies.
plexin-B1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PLXNB1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PLXNB1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PLXNB1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish plexin-B1 protein expression.
This CRISPR knockout system enables efficient generation of PLXNB1-deficient cell models for investigation of plexin-B1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PLXNB1 exon(s) critical for plexin-B1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PLXNB1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by plexin-B1 CRISPR/Cas9 KO Plasmid (h) and plexin-B1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PLXNB1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by plexin-B1 HDR Plasmid (h) and plexin-B1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PLXNB1 homology arms to support homology-directed repair at defined PLXNB1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.