Pit-1 Antibody (A-1): sc-55480

Pit-1 Antibody (A-1) is a mouse monoclonal IgG2a antibody that detects Pit-1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Pit-1, also known as growth hormone factor-1 (GHF-1), is a pivotal transcriptional regulator that plays a crucial role in the development of the anterior pituitary gland, where Pit-1 is essential for the differentiation and function of somatotropes, lactotropes, and thyrotropes. Pit-1 features a bipartite DNA binding domain, which is critical for activating the transcription of growth hormone and prolactin, thereby influencing growth and metabolic processes. Additionally, Pit-1 is expressed in two isoforms, Pit-1a and Pit-1b, which exhibit distinct trans-activation capabilities, further underscoring the complexity of regulatory functions. Mutations in the Pit-1 gene can lead to combined pituitary hormone deficiency (CPHD), affecting the production of growth hormone, prolactin, and thyroid-stimulating hormone, highlighting Pit-1′s significance in endocrine health. The gene encoding Pit-1 is located on human chromosome 3p11, making Pit-1 a critical target for studies related to pituitary function and associated disorders. Anti-Pit-1 antibody (A-1) is an invaluable tool for researchers investigating the intricate roles of this transcription factor in developmental biology and endocrine regulation.

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Pit-1 Antibody (A-1) References:

1. The tissue-specific transcription factor Pit-1/GHF-1 binds to the c-fos serum response element and activates c-fos transcription.  |  Gaiddon, C., et al. 1999. Mol Endocrinol. 13: 742-51. PMID: 10319324
2. Nucleotide sequence of the complementary DNA for human Pit-1/GHF-1.  |  Tatsumi, K., et al. 1992. Biochim Biophys Acta. 1129: 231-4. PMID: 1370379
3. The type III transporters (PiT-1 and PiT-2) are the major sodium-dependent phosphate transporters in the mice and human brains.  |  Inden, M., et al. 2016. Brain Res. 1637: 128-136. PMID: 26923164
4. Phosphate Overload Stimulates Inflammatory Reaction via PiT-1 and Induces Vascular Calcification in Uremia.  |  Ding, M., et al. 2022. J Ren Nutr. 32: 178-188. PMID: 34688540
5. SLC20a1/PiT-1 is required for chorioallantoic placental morphogenesis.  |  Correia-Branco, A., et al. 2023. Vasc Biol. 5: PMID: 36795703
6. Concomitant Prediction of the Ki67 and PIT-1 Expression in Pituitary Adenoma Using Different Radiomics Models.  |  Liu, F., et al. 2025. J Imaging Inform Med. 38: 394-409. PMID: 38750186
7. Negative regulation of expression of the pituitary-specific transcription factor GHF-1/Pit-1 by thyroid hormones through interference with promoter enhancer elements.  |  Sanchez-Pacheco, A., et al. 1995. Mol Cell Biol. 15: 6322-30. PMID: 7565785
8. Retinoic acid induces expression of the transcription factor GHF-1/Pit-1 in pituitary prolactin- and growth hormone-producing cell lines.  |  Sánchez-Pacheco, A., et al. 1995. Endocrinology. 136: 5391-8. PMID: 7588287
9. Pit-1/GHF-1 expression in pituitary adenomas: further analogy between human adenomas and rat SMtTW tumours.  |  Delhase, M., et al. 1993. J Mol Endocrinol. 11: 129-39. PMID: 8297469
10. GHF-1/Pit-1 functions as a cell-specific integrator of Ras signaling by targeting the Ras pathway to a composite Ets-1/GHF-1 response element.  |  Bradford, AP., et al. 1996. J Biol Chem. 271: 24639-48. PMID: 8798730

The preferred Pit-1 antibody is Pit-1 Antibody (G-2): sc-25258.