Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PIASy Double Nickase Plasmid (h): sc-403125-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PIASy Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PIASy Double Nickase Plasmid (h) and PIASy Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PIAS4. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PIASy Antibody (C-11): sc-166706
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PIASy Double Nickase Plasmid (h) | sc-403125-NIC | 20 µg | $410.00 |
PIAS4 encodes PIASy, a SUMO E3 ligase that modulates transcription factor activity and protein stability through SUMOylation. PIASy participates in regulation of JAK/STAT and NF-κB signaling, DNA damage responses, and chromatin-associated transcriptional control by influencing proteins such as p53 and other stress-responsive regulators. By tuning post-translational modification networks, PIASy impacts cell-cycle progression, apoptosis, and innate immune signaling programs. Dysregulated PIAS4/PIASy activity has been linked to altered inflammatory signaling and oncogenic transcriptional states, making it relevant for mechanistic studies of cancer biology and immune-associated pathways.
PIASy Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PIAS4 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PIAS4. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PIAS4 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PIAS4-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.