PHTF1 Antibody (PHTF1R8): sc-81111
- PHTF1 Antibody (PHTF1R8) is a mouse monoclonal IgG1 PHTF1 antibody provided at 100 µg/ml
- raised against a recombinant protein corresponding to an internal region of PHTF1 of human origin
- recommended for detection of PHTF1 of human origin by WB and IP
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for PHTF1 Antibody (PHTF1R8). This work is in progress.
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PHTF1 Antibody (PHTF1R8) is a mouse monoclonal IgG1 antibody that detects PHTF1 protein of human origin by western blotting (WB) and immunoprecipitation (IP). PHTF1 Antibody (PHTF1R8) is available as a non-conjugated anti-PHTF1 antibody (PHTF1R8). PHTF1, or putative homeodomain transcription factor 1, is a potential transcription regulator that plays a crucial role in cellular processes, particularly in spermatozoa development. PHTF1 is an integral, multipass membrane protein predominantly expressed in the testis and is associated with the endoplasmic reticulum. PHTF1 contains a basic helix-loop-helix (bHLH) domain, which is essential for function as a transcription factor, allowing DNA binding and gene expression regulation. During meiosis and spermiogenesis, PHTF1 is present in the cell, but releases from mature sperm cells within residual bodies, indicating involvement in sperm maturation. PHTF1 interacts with FEM1B, potentially recruiting FEM1B to the surface of the endoplasmic reticulum membrane, suggesting a role as a sequestering or anchoring protein. Two isoforms of PHTF1 exist due to alternative splicing, with isoform 2 being shorter and lacking specific amino acid residues. Anti-PHTF1 antibody (PHTF1R8) serves as an invaluable tool for researchers studying PHTF1′s role in reproductive biology and fertility implications.
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PHTF1 Antibody (PHTF1R8) References:
- Molecular characterization of a novel gene family (PHTF) conserved from Drosophila to mammals. | Manuel, A., et al. 2000. Genomics. 64: 216-20. PMID: 10729229
- Phtf1 is an integral membrane protein localized in an endoplasmic reticulum domain in maturing male germ cells. | Oyhenart, J., et al. 2003. Biol Reprod. 68: 1044-53. PMID: 12604659
- Expression, regulation, and immunolocalization of putative homeodomain transcription factor 1 (PHTF1) in rodent epididymis: evidence for a novel form resulting from proteolytic cleavage. | Oyhenart, J., et al. 2005. Biol Reprod. 72: 50-7. PMID: 15342352
- Putative homeodomain transcription factor 1 interacts with the feminization factor homolog fem1b in male germ cells. | Oyhenart, J., et al. 2005. Biol Reprod. 72: 780-7. PMID: 15601915
- Delineation of a 1Mb breakpoint region at 1p13 in Wilms tumors by fine-tiling oligonucleotide array CGH. | Natrajan, R., et al. 2007. Genes Chromosomes Cancer. 46: 607-15. PMID: 17370329
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PHTF1 Antibody (PHTF1R8) | sc-81111 | 100 µg/ml | $339.00 |