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western blotting image from recommended PGC1a (D-5) monoclonal antibody replacement. Image 390096 — Questo anticorpo policlonale è stato sospeso. Consulta il nostro anticorpo monoclonale PGC1a (D-5) raccomandato (sono riportati i dati del campione).

Questo anticorpo policlonale è stato sospeso. Consulta il nostro anticorpo monoclonale PGC1a (D-5) raccomandato (sono riportati i dati del campione).

Anticorpo PGC-1α (H-300): sc-13067

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PGC-1 alpha Antibody H-300 è un policlonale di coniglio IgG fornito in 200 µg/ml
epitopo corrispondente agli amminoacidi 1-300 mappati al N-terminus di PGC-1 di origine human
Anticorpo policlonale fuori produzione

Vedere citazioni di prodotto (192)

VEDI ANCHE...

Anticorpi addizionali PGC inclusi PGC-1 α e PGC-1 β

Informazioni ordini

PGC1a (D-5): sc-518025 [ Monoclonale raccomandato come sostituzione per PGC-1α (H-300) ]

Nome del prodotto	Codice del prodotto	UNITÀ	Prezzo	Quantità	Preferiti
PGC1a Anticorpo (D-5)	sc-518025	200 µg/ml	$316.00
PGC1a (D-5): m-IgG Fc BP-HRP Bundle	sc-531597	200 µg Ab; 10 µg BP	$354.00
PGC1a (D-5): m-IgGκ BP-HRP Bundle	sc-525331	200 µg Ab, 40 µg BP	$354.00
PGC1a (D-5): m-IgG_2a BP-HRP Bundle	sc-547825	200 µg Ab; 10 µg BP	$354.00
PGC1a Anticorpo (D-5) AC	sc-518025 AC	500 µg/ml, 25% agarose	$416.00
PGC1a Anticorpo (D-5) HRP	sc-518025 HRP	200 µg/ml	$316.00
PGC1a Anticorpo (D-5) FITC	sc-518025 FITC	200 µg/ml	$330.00
PGC1a Anticorpo (D-5) PE	sc-518025 PE	200 µg/ml	$343.00
PGC1a Anticorpo (D-5) Alexa Fluor^® 488	sc-518025 AF488	200 µg/ml	$357.00
PGC1a Anticorpo (D-5) Alexa Fluor^® 546	sc-518025 AF546	200 µg/ml	$357.00
PGC1a Anticorpo (D-5) Alexa Fluor^® 594	sc-518025 AF594	200 µg/ml	$357.00
PGC1a Anticorpo (D-5) Alexa Fluor^® 647	sc-518025 AF647	200 µg/ml	$357.00
PGC1a Anticorpo (D-5) Alexa Fluor^® 680	sc-518025 AF680	200 µg/ml	$357.00
PGC1a Anticorpo (D-5) Alexa Fluor^® 790	sc-518025 AF790	200 µg/ml	$357.00

If the product is specified for mice, can it work on rat tissue¿

Richiesta da: XimenaYri

Thank you for your question. PGC-1α(H-300) was recommended for detection of PGC-1α of mouse, rat and human origin. This antibody has been discontinued. We recommend you use one of our monoclonal alternatives, such as PGC-1α Anticuerpo (D-5): sc-518025, which is recommended for detection of PGC-1α of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA.

Risposta da: Tech Support Europe

Data di pubblicazione: 2021-11-09

what is the working concentration to be used

Richiesta da: Thara

Thank you for your question. Please refer to our protocols for the recommended concentrations for our antibodies. If you have any further questions, please contact Technical Service. https://www.scbt.com/resources/protocols

Risposta da: Technical Service 3

Data di pubblicazione: 2020-11-25

I am getting a band between 37 and 50 kDa with this antibody. Has someone else reported this?

Richiesta da: Kranti

Thank you for your question. The expected molecular weight of PGC-1α is 90 kDa, but do not have information regarding a smaller size. We recommend using our monoclonal alternative PGC-1α Antibody (D-5): sc-518025. Please contact our technical service if you require further information.

Risposta da: Technical Support

Data di pubblicazione: 2020-11-02

What is the expected molecular weight for bands?

Richiesta da: JJJJ55

Thank you for your question. The expected molecular weight of PGC-1α is 90 kDa. We recommend using our monoclonal alternative PGC-1α Antibody (D-5): sc-518025. Please contact our technical service if you require further information.

Risposta da: SCBT Heidelberg Support

Data di pubblicazione: 2019-03-07

I performed an immunoprecipitation using this antibody (PGC-1 alpha Antibody - H-300) and I had a problem since the heavy chain (HC, 55 kDa) of the IP antibody were also recognized.

Richiesta da: johnny86

Thank you for your question. This is rabbit polyclonal antibody, PGC-1α (H-300): sc-13067, is expected to detect the PGC-1α protein at approximately 91 kDa. The heavy chain of IgG is approximately 55 kDa. Therefore, observing the heavy chain IgG band from your immunoprecipitating antibody should not interfere with detection of your target protein. However, if you wish to not observe the heavy chain, then we would recommend using a different antibody for your Western blot. For example, a mouse monoclonal against PGC-1α. Then you could use an anti-mouse IgG secondary antibody, that would not recognize the heavy chain of the rabbit IgG primary used for immunoprecipitation.

Risposta da: Technical Support

Data di pubblicazione: 2018-08-17

I used this antibody on cells and get 2 bands very close together which one is pgc1 alpha upper or lower

Richiesta da: Joey88

Thank you for your question. We are sorry to hear that this antibody did not perform as expected. It is very difficult to say which band is which without having more information. Please reach out to our Technical Service Department by phone, e-mail or live chat. We are happy to help you troubleshoot the antibody and make sure you get the results you are looking for!

Risposta da: Tech Service

Data di pubblicazione: 2017-06-07

Rated 2 di 5 di Ted Chen da OKI bought this few months ago, it did not perform really well in western blot

Data di pubblicazione: 2018-09-28

Rated 5 di 5 di RahulK da Highly specific antibodyWe bought this antibody for use in western, IF and IHC and we are highly satisfied by its performance. WB- 1:1000, IF- 1:250, IHC- 1:200

Data di pubblicazione: 2017-03-09

Rated 5 di 5 di qiliqiang da Worked well in WBI used this antibody in WB (dilution 1:500), the band is wonderful.

Data di pubblicazione: 2016-11-01

Rated 5 di 5 di biosure da worked wellI used this antibody in WB (dilution 1:500), the band is wonderful.

Data di pubblicazione: 2016-10-31

Rated 1 di 5 di Thomas_Gantert da Multiple unspecific bands across antibodyMultiple unspecific bands across antibody dilutions (1:100-10:1000) and independent of protein loading (20-80ug). No band at expected size. Mouse liver tissues were used.

Data di pubblicazione: 2016-01-12

Rated 4 di 5 di Lin_Song da This has worked well in WBThis has worked well in WB.

Data di pubblicazione: 2015-11-04

Rated 5 di 5 di NN da This has worked well in WBThis has worked well in WB

Data di pubblicazione: 2015-10-27

Rated 4 di 5 di Dan da Used in IF and got good staining at 1:100 dilutionUsed in IF and got good staining at 1:100 dilution.

Data di pubblicazione: 2015-09-24

Anticorpo PGC-1α (H-300) Recensioni - pagina 2

Anticorpo PGC-1α (H-300) valutazione 4.0 di 5 di 16.

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Anticorpo PGC-1α (H-300): sc-13067

PGC1a Anticorpo (D-5)

PGC1a (D-5): m-IgG Fc BP-HRP Bundle

PGC1a (D-5): m-IgGκ BP-HRP Bundle

PGC1a (D-5): m-IgG2a BP-HRP Bundle

PGC1a Anticorpo (D-5) AC

PGC1a Anticorpo (D-5) HRP

PGC1a Anticorpo (D-5) FITC

PGC1a Anticorpo (D-5) PE

PGC1a Anticorpo (D-5) Alexa Fluor® 488

PGC1a Anticorpo (D-5) Alexa Fluor® 546

PGC1a Anticorpo (D-5) Alexa Fluor® 594

PGC1a Anticorpo (D-5) Alexa Fluor® 647

PGC1a Anticorpo (D-5) Alexa Fluor® 680

PGC1a Anticorpo (D-5) Alexa Fluor® 790

If the product is specified for mice, can it work on rat tissue&iquest;

what is the working concentration to be used

I am getting a band between 37 and 50 kDa with this antibody. Has someone else reported this?

What is the expected molecular weight for bands?

I performed an immunoprecipitation using this antibody (PGC-1 alpha Antibody - H-300) and I had a problem since the heavy chain (HC, 55 kDa) of the IP antibody were also recognized.

I used this antibody on cells and get 2 bands very close together which one is pgc1 alpha upper or lower

PGC1a (D-5): m-IgG_2a BP-HRP Bundle

PGC1a Anticorpo (D-5) Alexa Fluor^® 488

PGC1a Anticorpo (D-5) Alexa Fluor^® 546

PGC1a Anticorpo (D-5) Alexa Fluor^® 594

PGC1a Anticorpo (D-5) Alexa Fluor^® 647

PGC1a Anticorpo (D-5) Alexa Fluor^® 680

PGC1a Anticorpo (D-5) Alexa Fluor^® 790

If the product is specified for mice, can it work on rat tissue¿