Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PDK1 Double Nickase Plasmid (h): sc-401084-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PDK1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PDK1 Double Nickase Plasmid (h) and PDK1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PDK1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PDK1 Antibody (E-10): sc-515944
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PDK1 Double Nickase Plasmid (h) | sc-401084-NIC | 20 µg | $410.00 | |||
PDK1 Double Nickase Plasmid (h2) | sc-401084-NIC-2 | 20 µg | $410.00 |
Pyruvate dehydrogenase kinase 1 (PDK1) is a mitochondrial Ser/Thr kinase that phosphorylates and inhibits the pyruvate dehydrogenase complex, shifting carbon flux away from acetyl-CoA production and toward glycolytic metabolism. By restraining pyruvate entry into the TCA cycle, PDK1 contributes to metabolic flexibility during hypoxia and nutrient stress and is regulated downstream of pathways such as HIF-1 signaling. Altered PDK1 activity has been implicated in dysregulated glucose oxidation, mitochondrial function, and redox balance, processes frequently perturbed in cancer metabolism, diabetes-related metabolic remodeling, and other conditions characterized by altered oxidative phosphorylation. As a result, PDK1 is widely studied in contexts linking metabolic state to proliferation, survival, and stress adaptation.
PDK1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PDK1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PDK1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PDK1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PDK1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.