Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PDIR CRISPR/Cas9 KO Plasmid (h): sc-405741
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PDIR CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PDIR genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PDIR Antibody (B-9): sc-390862
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PDIR CRISPR/Cas9 KO Plasmid (h) | sc-405741 | 20 µg | $397.00 |
Overview
PDIA5 encodes the human protein disulfide isomerase-related protein PDIR, an endoplasmic reticulum–resident oxidoreductase that supports oxidative protein folding and disulfide bond isomerization. As a member of the protein disulfide isomerase family, PDIR contributes to ER proteostasis by facilitating maturation of secreted and membrane proteins and coordinating with chaperone networks engaged during the unfolded protein response. PDIA5 activity intersects with ER quality control, redox homeostasis, and ER-associated degradation (ERAD), processes frequently remodeled in cancer, metabolic stress, and neurodegenerative settings. Altered PDI-family function is commonly linked to heightened ER stress signaling and changes in secretory pathway capacity, making PDIA5 a useful node for mechanistic studies of redox-dependent folding.
PDIR CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PDIA5 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PDIA5 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PDIA5 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PDIR protein expression.
This CRISPR knockout system enables efficient generation of PDIA5-deficient cell models for investigation of PDIR signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PDIA5 exon(s) critical for PDIR function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PDIA5 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PDIR CRISPR/Cas9 KO Plasmid (h) and PDIR CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PDIA5 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PDIR HDR Plasmid (h) and PDIR HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PDIA5 homology arms to support homology-directed repair at defined PDIA5 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.