Date published: 2025-9-7

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Anticorpo PDGF Receptor alpha/PDGFRA (C-9): sc-398206

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Schede Tecniche
  • PDGF Receptor alpha/PDGFRA Antibody C-9 è un monoclonale di topo IgG1 κ PDGF Receptor alpha/PDGFRA antibody, citato in 47 pubblicazioni, fornito in 200 µg/ml
  • generato contro gli amminoacidi 951-1089 di PDGFR-α di origine human
  • PDGF Receptor alpha/PDGFRA Antibody (C-9) é raccomandato per il rilevamento di PDGFR-α di origine mouse, rat e human in WB, IP, IF, IHC(P) e ELISA; anche reattivo con ulteriori specie, incluse e equine, canine, bovine and porcine
  • PDGF Receptor alpha/PDGFRA Antibody (C-9) é disponibile coniugato con agarose per IP; HRP per WB, IHC(P) ed ELISA; sia con phycoerythrin o FITC per IF, IHC(P) e FCM
  • disponibile anche coniugato con Alexa Fluor® 488, Alexa Fluor® 546; Alexa Fluor® 594 o Alexa Fluor® 647 per IF, IHC (P) e FCM
  • disponibile anche coniugato con Alexa Fluor® 680 o Alexa Fluor® 790 per WB (NIR), IF e FCM
  • Contattaci per ricevere un FREE 10 µg sample di PDGF Receptor alpha/PDGFRA (C-9): sc-398206.
  • m-IgG Fc BP-HRP e m-IgG1 BP- HRP sono i reagenti di rivelazione secondaria preferiti per PDGF Receptor alpha/PDGFRA anticorpo (C-9) per le applicazioni WB and IHC(P). Questi reagenti sono ora offerti in bundle con PDGF Receptor alpha/PDGFRA Anticorpo (C-9) (vedi informazioni per l'ordine sotto).

LINK RAPIDI

VEDI ANCHE...

L'anticorpo PDGFR-α (C-9) è un anticorpo monoclonale di topo IgG1 κ PDGFR-alfa (designato anche come anticorpo PDGFRA o anticorpo CD140a) che rileva la proteina PDGFR-alfa di origine murina, ratta e umana mediante WB, IP, IF, IHC(P) ed ELISA. L'anticorpo PDGFR-α (C-9) è disponibile sia nella forma di anticorpo anti-PDGFR-alfa non coniugato, sia in molteplici forme coniugate di anticorpo anti-PDGFR-alfa, tra cui agarosio, HRP, PE, FITC e molteplici coniugati Alexa Fluor®. Il fattore di crescita derivato dalle piastrine (PDGF) è un mitogeno per le cellule mesenchimali e glia-derivate. Il PDGF è costituito da due catene, A e B, che si dimerizzano per formare isoforme funzionalmente distinte PGDF-AA, PDGF-AB e PDGF-BB. Queste tre isoforme si legano con diverse affinità a due tipi di recettori, PDGFR-α e -β, dotati di domini proteici tirosin-chinasici. PDGFR-α può legarsi sia alla subunità A che alla subunità B di PDGF, mentre PDGFR-β può legare solo la subunità B. Il legame con il ligando promuove l'omo- o l'eterodimerizzazione dei recettori PDGF in modo specifico. PDGF-AA induce la dimerizzazione di due recettori α, PDGF-AB induce la dimerizzazione di αα e αβ e PDGF-BB induce la formazione di tre tipi di dimeri, αα, αβ e β. I geni che codificano il PDGFR-α e -β mappano sul cromosoma umano 4q12 e 5q33.1, rispettivamente. La traslocazione del gene PDGFR-β con il gene TEL è legata alla leucemia mielomonocitica cronica (CMML), una sindrome mielodisplastica, e dimostra il potenziale oncogeno dei recettori PDGF.

Solo per ricerca in vitro. Non destinato ad uso Diagnostico o Terapeutico.

Alexa Fluor® è un marchio registrato di Molecular Probes Inc., OR., USA

LI-COR® e Odyssey® sono marchi registrati di LI-COR Biosciences

Informazioni ordini

Nome del prodottoCodice del prodottoUNITÀPrezzoQuantitàPreferiti

PDGF Receptor alpha/PDGFRA Anticorpo (C-9)

sc-398206
200 µg/ml
$316.00

PDGF Receptor alpha/PDGFRA (C-9): m-IgG Fc BP-HRP Bundle

sc-527749
200 µg Ab; 10 µg BP
$354.00

PDGF Receptor alpha/PDGFRA (C-9): m-IgG1 BP-HRP Bundle

sc-533122
200 µg Ab; 20 µg BP
$354.00

PDGF Receptor alpha/PDGFRA Anticorpo (C-9) AC

sc-398206 AC
500 µg/ml, 25% agarose
$416.00

PDGF Receptor alpha/PDGFRA Anticorpo (C-9) HRP

sc-398206 HRP
200 µg/ml
$316.00

PDGF Receptor alpha/PDGFRA Anticorpo (C-9) FITC

sc-398206 FITC
200 µg/ml
$330.00

PDGF Receptor alpha/PDGFRA Anticorpo (C-9) PE

sc-398206 PE
200 µg/ml
$343.00

PDGF Receptor alpha/PDGFRA Anticorpo (C-9) Alexa Fluor® 488

sc-398206 AF488
200 µg/ml
$357.00

PDGF Receptor alpha/PDGFRA Anticorpo (C-9) Alexa Fluor® 546

sc-398206 AF546
200 µg/ml
$357.00

PDGF Receptor alpha/PDGFRA Anticorpo (C-9) Alexa Fluor® 594

sc-398206 AF594
200 µg/ml
$357.00

PDGF Receptor alpha/PDGFRA Anticorpo (C-9) Alexa Fluor® 647

sc-398206 AF647
200 µg/ml
$357.00

PDGF Receptor alpha/PDGFRA Anticorpo (C-9) Alexa Fluor® 680

sc-398206 AF680
200 µg/ml
$357.00

PDGF Receptor alpha/PDGFRA Anticorpo (C-9) Alexa Fluor® 790

sc-398206 AF790
200 µg/ml
$357.00

Do you have any references to the use of conjugated PDGFRa antibody in flow cytometry?

Richiesta da: JustynaJ
Thank you for your question. We do not have internal image data for flow cytometry use of this antibody yet, but the antibody was used in FCM in publication PMID 30057110.
Risposta da: Tech Support Europe
Data di pubblicazione: 2021-12-17

PDGFR-a(C-9):sc-398206 antibody did not work in the tissue? immunofluorescence (dilution 1:50)

Richiesta da: yaoshen
Thank you for your question. Please contact our Technical Service team for assistance and troubleshooting advice.
Risposta da: Tech Support Europe
Data di pubblicazione: 2021-01-25

Hi, I was wondering if this antibody has been tested to cross-react with PDGFRbeta? We would like to buy a highly specific PDGFRalpha antibody for use on a Western blot.

Richiesta da: NataPa
Thank you for your question. The antibody was raised against amino acids 951-1089 of PDGFR- of human origin with accession number https://www.ncbi.nlm.nih.gov/protein/129892 The exact binding region of this antibody within the antigen has not been mapped. This antibody has not been tested experimentally for potential cross-reactivity under denaturing conditions in a Western Blot against different proteins. You might want to align the antigen sequence against the protein form of interest in order to determine potential reactivity. Please contact our Technical Service by phone or email or by Live Chat if you have any further questions.
Risposta da: SCBT Heidelberg Support
Data di pubblicazione: 2019-03-04

Hi, can you please advise if for staining on cells it is necessary to permeabilise cells with Triton-X? Is the antibody binding to an intra- or extra-cellular site? Many thanks in advance.

Richiesta da: Heart_researcher
Thank you for your question. PDGFR-α Antibody (C-9) is a mouse monoclonal IgG1 raised against amino acids 951-1089 of PDGFR-α of human origin. According to UniProt, this corresponds with a cytoplasmic region of the protein (https://www.ncbi.nlm.nih.gov/protein/P16234). If you are using a formalin-based fixative (e.g., PFA) then we do recommend including a cell permeabilization treatment. After fixing the cells, wash in three changes of PBS. Then incubate for 3 minutes in a solution of .01% Triton X-100 in PBS. Followed by a wash in three changes of PBS. Below is a link to our recommended protocol for immunofluorescence cell staining with this antibody. https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Risposta da: Technical Support
Data di pubblicazione: 2024-10-03

Can I use PDGFR-α (C-9): sc-398206 mouse monoclonal antibody for immunohistochemical staining of mouse tissue?

Richiesta da: jenni
Yes. We recommend using a directly conjugated monoclonal antibody. NCAM (123C3): sc-7326 monoclonal antibody is available directly conjugated to either HRP (sc-398206 HRP), phycoerythrin (sc-398206 PE), fluorescein (sc-398206 FITC), Alexa Fluor® 488 (sc-398206 AF488) or Alexa Fluor® 647 (sc-398206 AF647)
Risposta da: Technical Support 4
Data di pubblicazione: 2017-01-09

请问有没有小包装的?还有我想要NG2的大鼠的小包装抗体,请问有没有?请尽快回复

Richiesta da: 穆娇66
感谢您的提问。sc-398206为200 µg/ml,1 ml装。但如果您感兴趣,我们可以提供一支10 µg/50 µl的小样供您试用。如需试用,请联系我们的技术支持部门:电话(+86) 021-60936351,邮箱asia@scbio.cn。 Thank you for your question. We provide sc-398206 at 200 µg/ml. If you are interested in testing a 10 µg/50 µl free sample, please contact our Technical Services department at (+86) 021-60936351 or asia@scbio.cn.
Risposta da: Technical Support 12
Data di pubblicazione: 2017-01-06

Bonjour, Je voulais savoir si il était possible d'obtenir un échantillon de l'anticorps PDGFR-α (C-9) sc-398206 pour tester son efficacité sur nos tissus de poulet. D'avance merci. Cordialement

Richiesta da: cpraud
Merci pour votre question. L'anticorps monoclonal PDGFR-α (C-9) sc-398206 a été élaboré contre les acides aminés 951-1089 de PDGFR-α d'origine humaine, correspondant au numéro d'accession NCBI P16234. https://www.ncbi.nlm.nih.gov/protein/P16234 L'anticorps est recommandé pour la détection de la protéine humaine, de souris, de rat, équine, canine, bovine et porcine. Notre laboratoire n'a malheureusement pas testé la réactivité avec PDGFR-α de poulet (aviaire). L'homologie entre les séquences des protéines de poulet et humaine est relativement élevée et il est très probable que l'anticorps réagisse aussi avec PDGFR-α aviaire. Cependant, l'épitope exact de cet anticorps monoclonal n'a pas été caractérisé. C'est pourquoi, l'anticorps peut ou non être adapté aux expériences souhaitées avec des échantillons de poulet. Pour recevoir un échantillon gratuit de 10μg, veuillez nous contacter par téléphone au +49 6221 45030 ou par email à l'adresse france@scbt.com et indiquez-nous une adresse complète de livraison. -- Thank you for the question. The monoclonal PDGFR-α (C-9) antibody sc-398206 was raised against amino acids 951-1089 of PDGFR-α of human origin with corresponding NCBI protein accession number P16234. https://www.ncbi.nlm.nih.gov/protein/P16234 The antibody is recommended for the detection of the protein in human, mouse, rat, equine, canine, bovine and porcine samples. Our lab unfortunately has not yet tested reactivity with the chicken (avian) PDGFR-α. The homology between the chicken and human protein sequences is relatively high and it seems very likely the antibody will also pick-up the avian PDGFR-α. However, the exact binding epitope for this monoclonal antibody has never been mapped. Therefore, the antibody might or might not be suitable for the desired experiment with Samples from chicken origin. In order to receive a free 10µg Sample, please contact us by phone +49 6221 45030 or email france@scbt.com and provide your full shipping address.
Risposta da: Technical Support Europe
Data di pubblicazione: 2016-10-25
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Rated 5 di 5 di da 条带很亮本月订购的这个抗体,条带比较单一,特异性比较好,亮度也不错的,比之前购买的ab公司的要划算很多,导师也比较满意。
Data di pubblicazione: 2022-09-06
Rated 5 di 5 di da Western Blot and IHC on canine samplesWorked well on dog samples; 1:500 in Western Blot and 1:200 in IHC on FFPE tissue
Data di pubblicazione: 2022-07-13
Rated 5 di 5 di da Detects mouse PDGFRaOne clear band of correct molecular weight. No non-specific binding.
Data di pubblicazione: 2021-12-02
Rated 1 di 5 di da does not worki tested the antibody with various concentrations and with different sample but we we couldn't get a well-defined band.
Data di pubblicazione: 2021-07-13
Rated 1 di 5 di da Does no work and not good costumer serviceIt did not work in formalin fixed/paraffin embedded human brain, dilution 1/40. NOTE: Other antibodies tested in the same tissue worked fine. Costumer service keeps asking questions for more than 2 weeks and no replacement has been issued. Disappointed.
Data di pubblicazione: 2020-11-09
Rated 5 di 5 di da 発現確認できましたPDGFRA発現の確認のため、本製品のサンプルをオーダーしました。目的を達成する事が出来て満足です。
Data di pubblicazione: 2020-05-31
Rated 4 di 5 di da ImmunofluoresceI used the C-9 Santa Cruz antibody in an MGC culture in order to detect Oligodendrocyte precursor cells. The Signal was clear and allowed for easy distinction among the other cells types in the culture.
Data di pubblicazione: 2018-04-30
Rated 1 di 5 di da Did not work at 1:500 dilution for WB analysisTried 1:500 and 1:1000 dilution in human and canine bladder cancer cell lines - no signal detected by WB analysis
Data di pubblicazione: 2018-04-03
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Anticorpo PDGF Receptor alpha/PDGFRA (C-9) valutazione 4.0 di 5 di 20.
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