Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
paxillin Double Nickase Plasmid (h): sc-416733-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- paxillin Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- paxillin Double Nickase Plasmid (h) and paxillin Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PXN. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: paxillin Antibody (B-2): sc-365379
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
paxillin Double Nickase Plasmid (h) | sc-416733-NIC | 20 µg | $410.00 | |||
paxillin Double Nickase Plasmid (h2) | sc-416733-NIC-2 | 20 µg | $410.00 |
PXN encodes paxillin, a multi-domain focal adhesion adaptor that coordinates integrin signaling with actin cytoskeletal remodeling by scaffolding kinases and GTPase regulators at sites of cell–matrix contact. Through interactions with FAK/PTK2, SRC family kinases, CRK, and ARF/RHO pathway components, paxillin helps regulate adhesion turnover, cell spreading, migration, and mechanotransduction. PXN participates in pathways linking extracellular matrix cues to MAPK and PI3K signaling outputs that influence proliferation and survival programs. Altered paxillin expression, phosphorylation, or focal-adhesion dynamics has been associated with invasive behavior and metastatic potential across multiple tumor contexts, motivating its use in models of cell motility and adhesion-dependent signaling.
paxillin Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PXN locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PXN. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PXN function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PXN-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.