Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PAR4 CRISPR/Cas9 KO Plasmid (m): sc-431134
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PAR4 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PAR4 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PAR4 Antibody (A-10): sc-1666
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PAR4 CRISPR/Cas9 KO Plasmid (m) | sc-431134 | 20 µg | $397.00 |
Overview
Mouse Pawr encodes PAR4 (prostate apoptosis response 4), a proline-rich, leucine zipper–containing protein that modulates transcriptional programs controlling cell fate decisions. PAR4 promotes apoptotic signaling and can antagonize pro-survival pathways, including interactions that influence NF-κB activity and protein kinase signaling cascades such as PKC-dependent responses. It has been linked to cellular stress responses, differentiation, and cytoskeletal regulation through its capacity to scaffold signaling complexes and regulate gene expression. Dysregulated PAWR/PAR4 activity has been associated with altered apoptosis thresholds in cancer biology and with inflammatory and neurodegeneration-relevant processes where stress-induced cell death and survival signaling are perturbed.
PAR4 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Pawr gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Pawr together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Pawr open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PAR4 protein expression.
This CRISPR knockout system enables efficient generation of Pawr-deficient cell models for investigation of PAR4 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Pawr exon(s) critical for PAR4 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Pawr genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PAR4 CRISPR/Cas9 KO Plasmid (m) and PAR4 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Pawr locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PAR4 HDR Plasmid (m) and PAR4 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Pawr homology arms to support homology-directed repair at defined Pawr target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.