PAR-4 CRISPR/Cas9 KO Plasmid (m): sc-420267
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PAR-4 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PAR-4 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PAR-4 CRISPR/Cas9 KO Plasmid (m) | sc-420267 | 20 µg | $397.00 |
Overview
Mouse F2rl3 encodes protease-activated receptor-4 (PAR-4), a G protein–coupled receptor activated by serine proteases such as thrombin through proteolytic unmasking of a tethered ligand. PAR-4 couples primarily to Gq and G12/13 signaling to drive intracellular Ca2+ mobilization, RhoA/ROCK-dependent cytoskeletal remodeling, and downstream MAPK and NF-κB pathway responses that shape platelet activation, inflammatory signaling, and vascular homeostasis. In immune and vascular contexts, F2rl3/PAR-4 activity contributes to cell adhesion and barrier regulation and is frequently studied in models of thrombosis, inflammation, and cardiometabolic stress. Altered PAR-4 signaling has also been linked to tumor-associated microenvironmental cues, supporting its relevance to mechanistic studies in hemostasis–inflammation cross-talk.
PAR-4 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the F2rl3 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the F2rl3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the F2rl3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PAR-4 protein expression.
This CRISPR knockout system enables efficient generation of F2rl3-deficient cell models for investigation of PAR-4 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting F2rl3 exon(s) critical for PAR-4 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple F2rl3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PAR-4 CRISPR/Cas9 KO Plasmid (m) and PAR-4 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the F2rl3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PAR-4 HDR Plasmid (m) and PAR-4 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by F2rl3 homology arms to support homology-directed repair at defined F2rl3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.