Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Paf1 Double Nickase Plasmid (h): sc-417797-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Paf1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Paf1 Double Nickase Plasmid (h) and Paf1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PAF1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Paf1 Antibody (E-7): sc-514491
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Paf1 Double Nickase Plasmid (h) | sc-417797-NIC | 20 µg | $410.00 |
Human PAF1 encodes Paf1, a core component of the RNA polymerase II–associated PAF1 complex that coordinates transcription elongation with co-transcriptional chromatin regulation. Through interactions with elongation factors and histone modification machinery, Paf1 helps regulate H2B monoubiquitination and downstream H3K4/H3K79 methylation, linking gene expression to epigenetic state. The PAF1 complex also contributes to RNA processing and genome stability, including roles in replication–transcription conflicts and DNA damage responses. Dysregulated PAF1 complex activity has been associated with altered transcriptional programs observed in multiple cancers and other proliferative or stress-related cellular contexts, making PAF1 a useful target for mechanistic studies of transcription and chromatin dynamics.
Paf1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PAF1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PAF1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PAF1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PAF1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.