Anti-p53 Antibody Pab 246 è un monoclonale di topo IgG1 (kappa light chain) p53 antibody fornito in 200 µg/ml
epitopo mapping sui residui aminoacidici 88-93 di mouse p53
p53 Antibody (Pab 246) é raccomandato per il rilevamento di p53 di origine mouse e rat in WB, IP e IF; potrebbe cross-reagire con wild type p53; non cross-reattivo con mutant p53 under non-denaturing conditions
p53 Antibody (Pab 246) é disponibile coniugato con agarose per IP; HRP per WB, IHC(P) ed ELISA; sia con phycoerythrin o FITC per IF, IHC(P) e FCM
disponibile anche coniugato con Alexa Fluor® 488, Alexa Fluor® 546; Alexa Fluor® 594 o Alexa Fluor® 647 per IF, IHC (P) e FCM
disponibile anche coniugato con Alexa Fluor® 680 o Alexa Fluor® 790 per WB (NIR), IF e FCM
Vedi m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), le nostre alternative ricombinanti ai reagenti secondari convenzionali anti-mouse IgG altamente raccomandate.
Contattaci per ricevere un FREE 10 µg sample di p53 (Pab 246): sc-100.
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p53, a DNA-binding, oligomerization domain- and transcription activation domain-containing tumor suppressor, upregulates growth arrest and apoptosis-related genes in response to stress signals, thereby influencing programmed cell death, cell differentiation, and cell cycle control mechanisms. p53 localizes to the nucleus, yet can be chaperoned to the cytoplasm by the negative regulator, MDM2. MDM2 is an E3 ubiquitin ligase that is upregulated in the presence of active p53, where it poly-ubiquitinates p53 for proteasome targeting. p53 fluctuates between latent and active DNA-binding conformations and is differentially activated through posttranslational modifications, including phosphorylation and acetylation. Mutations in the DNA-binding domain (DBD) of p53, amino acids 110-286, can compromise energetically-favorable association with cis elements and are implicated in several human cancers.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Thank you for your question. Yes, this p53 (Pab 246) antibody, sc-100, is recommended and warrantied for IP applications, which includes ChIP.
Risposta da: Tech Service
Data di pubblicazione: 2020-01-06
I am going to do a Western blot experiment using the LiCor/Odyssey system. Which secondary antibody do you recommend using with p53 (Pab 246): sc-100?
Richiesta da: TinTin
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems.
A complete list of available binding proteins is available on our website here:
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