Phosphotyrosine Antibody (PY99): sc-7020

p-Tyr Antibody (PY99) is a mouse monoclonal IgG2b kappa light chain antibody that detects phosphorylated tyrosine protein across broad species through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. p-Tyr (PY99) antibody comes in non-conjugated forms and various conjugates, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® options, offering flexibility for diverse experimental needs. Protein tyrosine phosphorylation plays a crucial role in cellular signaling pathways, serving as a key regulatory mechanism in numerous biological processes, including cell growth, differentiation, and metabolism. Phosphorylation of tyrosine residues occurs primarily through protein tyrosine kinases, which include receptor tyrosine kinases and non-receptor tyrosine kinases, with the Src family representing a classic example of the latter. These kinases typically localize to the plasma membrane′s inner surface, where they interact with surface receptors to transmit signals within cells. When receptor tyrosine kinases bind to ligands, they undergo dimerization and autophosphorylation, creating specific phosphotyrosine sites that dock downstream signaling molecules. This complex network highlights the significance of studying phosphotyrosine modifications, making anti-p-Tyr antibody (PY99) essential for researchers studying and purifying proteins containing phosphotyrosyl residues, ultimately advancing cellular signaling research.

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Phosphotyrosine Antibody (PY99) References:

1. Purification of the platelet-derived growth factor receptor by using an anti-phosphotyrosine antibody.  |  Daniel, TO., et al. 1985. Proc Natl Acad Sci U S A. 82: 2684-7. PMID: 2581254
2. Purification and characterization of a protein-tyrosine kinase encoded by the Abelson murine leukemia virus.  |  Foulkes, JG., et al. 1985. J Biol Chem. 260: 8070-7. PMID: 2989275
3. Phosphotyrosine profiling of human cerebrospinal fluid.  |  Sathe, G., et al. 2018. Clin Proteomics. 15: 29. PMID: 30220890
4. Blockade of phosphotyrosine pathways suggesting SH2 superbinder as a novel therapy for pulmonary fibrosis.  |  Wang, M., et al. 2022. Theranostics. 12: 4513-4535. PMID: 35832075
5. Tumor promoters block tyrosine-specific phosphorylation of the epidermal growth factor receptor.  |  Friedman, B., et al. 1984. Proc Natl Acad Sci U S A. 81: 3034-8. PMID: 6328489
6. Signalling by receptor tyrosine kinases.  |  Fantl, WJ., et al. 1993. Annu Rev Biochem. 62: 453-81. PMID: 7688944
7. Regulation of signal transduction and signal diversity by receptor oligomerization.  |  Lemmon, MA. and Schlessinger, J. 1994. Trends Biochem Sci. 19: 459-63. PMID: 7855887
8. Incorporation of leucocyte GPI-anchored proteins and protein tyrosine kinases into lipid-rich membrane domains of COS-7 cells.  |  Cebecauer, M., et al. 1998. Biochem Biophys Res Commun. 243: 706-10. PMID: 9500981
9. T cell receptor signalling results in rapid tyrosine phosphorylation of the linker protein LAT present in detergent-resistant membrane microdomains.  |  Brdiĉka, T., et al. 1998. Biochem Biophys Res Commun. 248: 356-60. PMID: 9675140

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Phosphotyrosine Antibody (PY99) has 2,689 citations in a variety of scientific publications.

Primary antibodies like Anti-Phosphotyrosine Antibody (PY99) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Phosphotyrosine Antibody (PY99) Specifications

Alternate Product Name: phosphotyrosine

Product Clone Name: PY99

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: N/A

Epitope Target: p-Tyr

Epitope Species:

Product Reactivity/Detection: phosphotyrosine-containing proteins

Species Reactivity/Detection: broad species

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2,689 citations in various scientific publications

Available Conjugates/Forms for Phosphotyrosine Antibody (PY99): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, AP: Alkaline Phosphatase, B: Biotin, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin