Anticorpo p-PYK2 (14F6) raccomandato per il rilevamento di Tyr 402 phosphorylated PYK2 di origine mouse, rat e human in WB e IP

Anticorpo p-PYK2 (14F6): sc-81512

Anticorpo p-PYK2 (14F6) valutazione 3.0 di 5 di 1.
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Schede tecniche

    • p-PYK2 Antibody 14F6 è un monoclonale di topo IgG1, citato in 3 pubblicazioni, fornito in 50 µg/0.5 ml
    • raised against a synthetic phosphopeptide corresponding to amino acid residues surrounding tyrosine 402 of PYK2 of human origin
    • raccomandato per il rilevamento di Tyr 402 phosphorylated PYK2 di origine mouse, rat e human in WB e IP; non cross-reattivo con non-phosphorylated form of PYK2 or with unrelated phosphorylation sites
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for p-PYK2 Antibody (14F6). This work is in progress.
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I am using p-PYK2 (14F6): sc-81512 antibody for immunoprecipitation, how much antibody should I use?

Richiesta da: DefinitelyNotMatt
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Risposta da: Technical Support
Data di pubblicazione: 2017-03-24
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Rated 3 di 5 di da Worked with transfected lysate by WB with Worked with transfected lysate by WB with some background -SCBT QC
Data di pubblicazione: 2014-04-17
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