p-paxillin Antibody (D-10): sc-271980

p-paxillin Antibody (D-10) is a mouse monoclonal IgG1 antibody that detects paxillin in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-paxillin antibody (D-10) specifically recognizes the phosphorylated form of paxillin, a crucial cytoskeletal protein that plays a significant role in cellular adhesion and signaling. Paxillin is primarily located at focal adhesions, which are dynamic structures that connect the extracellular matrix to the actin cytoskeleton, facilitating communication between the cell and its environment. This localization is vital for various cellular processes, including migration, proliferation, and survival, as paxillin allows cells to respond to external signals and mechanical forces. Furthermore, paxillin′s interaction with focal adhesion kinase (FAK) is essential for the regulation of tyrosine phosphorylation, which is a key event in the signaling pathways activated by growth factors and oncogenes. Phosphorylation of paxillin not only enhances binding to FAK but also promotes the recruitment of other signaling molecules, thereby amplifying the cellular response to adhesion and stress. Paxillin monoclonal antibody (D-10) enables researchers to gain insights into the mechanisms of cell adhesion and migration, which are critical in understanding cancer metastasis and tissue repair.

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p-paxillin Antibody (D-10) References:

1. The explorations of dynamic interactions of paxillin at the focal adhesions.  |  Aziz, AUR., et al. 2022. Biochim Biophys Acta Proteins Proteom. 1870: 140825. PMID: 35926716
2. Paxillin regulates Rab5-mediated vesicle motility through modulating microtubule acetylation.  |  Zehrbach, NM., et al. 2023. Mol Biol Cell. 34: ar65. PMID: 37043310
3. Direct active Fyn-paxillin interaction regulates vascular smooth muscle cell migration.  |  Zhang, Y., et al. 2023. J Smooth Muscle Res. 59: 58-66. PMID: 37438114
4. Paxillin knockout in mouse granulosa cells increases fecundity†.  |  Vann, K., et al. 2023. Biol Reprod. 109: 669-683. PMID: 37552051
5. Phenotype screens of murine pancreatic cancer identify a Tgf-α-Ccl2-paxillin axis driving human-like neural invasion.  |  Wang, X., et al. 2023. J Clin Invest. 133: PMID: 37607005
6. Paxillin regulates liver fibrosis via actin polymerization and ERK activation in hepatic stellate cells.  |  Hijazi, N., et al. 2023. J Cell Sci. 136: PMID: 37667902
7. Paxillin/HDAC6 regulates microtubule acetylation to promote directional migration of keratinocytes driven by electric fields.  |  Liu, L., et al. 2024. Biochim Biophys Acta Mol Cell Res. 1871: 119628. PMID: 37949303
8. Paxillin participates in the sphingosylphosphorylcholine-induced abnormal contraction of vascular smooth muscle by regulating Rho-kinase activation.  |  Zhang, Y., et al. 2024. Cell Commun Signal. 22: 58. PMID: 38254202
9. Paxillin phase separation promotes focal adhesion assembly and integrin signaling.  |  Liang, P., et al. 2024. J Cell Biol. 223: PMID: 38466167
10. Paxillin regulates androgen receptor expression associated with granulosa cell focal adhesions.  |  Weidner, AE., et al. 2024. Mol Hum Reprod. 30: PMID: 38718206

The preferred paxillin antibody is paxillin Antibody (B-2): sc-365379.