p-NFATc3 Antibody (C-3): sc-365786
- p-NFATc3 Antibody (C-3) is a mouse monoclonal IgG1 κ, cited in 4 publications, provided at 200 µg/ml
- Anti-p-NFATc3 Antibody (C-3) is recommended for detection of Ser 240 phosphorylated NFATc3 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-p-NFATc3 Antibody (C-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for p-NFATc3 Antibody (C-3) for WB applications. These reagents are now offered in bundles with p-NFATc3 Antibody (C-3) (see ordering information below).
p-NFATc3 Antibody (C-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects Ser 240 phosphorylated NFATc3 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-p-NFATc3 antibody (C-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. NFATc3, a member of the NFAT (nuclear factor of activated T cells) family, plays a crucial role in regulating gene expression in response to calcium signaling in immune cells. NFATc3 is primarily located in the cytosol and nucleus, where NFATc3 undergoes a significant conformational change upon dephosphorylation by calcineurin, allowing translocation into the nucleus and activation of cytokine gene transcription. This translocation is vital for T cell activation and differentiation, making NFATc3 an essential player in the immune response. The ability of NFAT proteins to form complexes with AP-1 proteins, such as Fos and Jun, further underscores their importance in immune function regulation. p-NFATc3 (C-3) monoclonal antibody enables researchers to gain insights into NFATc3 activation dynamics and implications in various immunological processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
p-NFATc3 Antibody (C-3) References:
- Recognition of NFATp/AP-1 composite elements within genes induced upon the activation of immune cells. | Kel, A., et al. 1999. J Mol Biol. 288: 353-76. PMID: 10329147
- NFAT5, a constitutively nuclear NFAT protein that does not cooperate with Fos and Jun. | Lopez-Rodríguez, C., et al. 1999. Proc Natl Acad Sci U S A. 96: 7214-9. PMID: 10377394
- Enhanced NFATc1 nuclear occupancy causes T cell activation independent of CD28 costimulation. | Pan, M., et al. 2007. J Immunol. 178: 4315-21. PMID: 17371988
- Hypoxia-induced NFATc3 deSUMOylation enhances pancreatic carcinoma progression. | Tong, Y., et al. 2022. Cell Death Dis. 13: 413. PMID: 35484132
- Isolation of two new members of the NF-AT gene family and functional characterization of the NF-AT proteins. | Hoey, T., et al. 1995. Immunity. 2: 461-72. PMID: 7749981
- Characterization of a new isoform of the NFAT (nuclear factor of activated T cells) gene family member NFATc. | Park, J., et al. 1996. J Biol Chem. 271: 20914-21. PMID: 8702849
- Expression of NFAT-family proteins in normal human T cells. | Lyakh, L., et al. 1997. Mol Cell Biol. 17: 2475-84. PMID: 9111316
- Transcription factors of the NFAT family: regulation and function. | Rao, A., et al. 1997. Annu Rev Immunol. 15: 707-47. PMID: 9143705
- Distinct NFAT family proteins are involved in the nuclear NFAT-DNA binding complexes from human thymocyte subsets. | Amasaki, Y., et al. 1998. J Immunol. 160: 2324-33. PMID: 9498773
- The transcription factor NF-ATc is essential for cardiac valve formation. | Ranger, AM., et al. 1998. Nature. 392: 186-90. PMID: 9515964
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
p-NFATc3 Antibody (C-3) | sc-365786 | 200 µg/ml | $322.00 | |||
p-NFATc3 Antibody (C-3): m-IgG Fc BP-HRP Bundle | sc-529466 | 200 µg Ab; 10 µg BP | $361.00 | |||
p-NFATc3 Antibody (C-3): m-IgGκ BP-HRP Bundle | sc-522367 | 200 µg Ab, 40 µg BP | $361.00 | |||
p-NFATc3 Antibody (C-3) AC | sc-365786 AC | 500 µg/ml, 25% agarose | $424.00 | |||
p-NFATc3 Antibody (C-3) HRP | sc-365786 HRP | 200 µg/ml | $322.00 | |||
p-NFATc3 Antibody (C-3) FITC | sc-365786 FITC | 200 µg/ml | $336.00 | |||
p-NFATc3 Antibody (C-3) PE | sc-365786 PE | 200 µg/ml | $349.00 | |||
p-NFATc3 Antibody (C-3) Alexa Fluor® 488 | sc-365786 AF488 | 200 µg/ml | $364.00 | |||
p-NFATc3 Antibody (C-3) Alexa Fluor® 546 | sc-365786 AF546 | 200 µg/ml | $364.00 | |||
p-NFATc3 Antibody (C-3) Alexa Fluor® 594 | sc-365786 AF594 | 200 µg/ml | $364.00 | |||
p-NFATc3 Antibody (C-3) Alexa Fluor® 647 | sc-365786 AF647 | 200 µg/ml | $364.00 | |||
p-NFATc3 Antibody (C-3) Alexa Fluor® 680 | sc-365786 AF680 | 200 µg/ml | $364.00 | |||
p-NFATc3 Antibody (C-3) Alexa Fluor® 790 | sc-365786 AF790 | 200 µg/ml | $364.00 | |||
p-NFATc3 (C-3) Neutralizing Peptide | sc-365786 P | 100 µg/0.5 ml | $69.00 |