p-C3G Antibody (G-2): sc-365994

p-C3G Antibody (G-2) is a mouse monoclonal IgM antibody that detects p-C3G protein of mouse origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-p-C3G antibody (G-2) is available as the non-conjugated form. p-C3G protein, also known as phospho-RAPGEF1, plays a crucial role in regulating Ras signaling pathways, which are essential for various cellular processes, including cell growth, differentiation, and survival. Ras proteins function as molecular switches, toggling between an active GTP-bound state and an inactive GDP-bound state, and dysregulation can lead to oncogenic transformations. C3G acts as a guanine nucleotide exchange factor (GEF) for Ras, facilitating GDP for GTP exchange, thereby promoting active signaling state. C3G interaction with Crk proto-oncogene product underscores importance in signaling cascade that regulates Ras activity. Additionally, C3G gene is located on human chromosome 9q34.13, near c-Abl proto-oncogene, which further highlights potential role in cancer biology. Understanding p-C3G function and regulatory mechanisms is vital for elucidating complexities of Ras signaling and implications in tumorigenesis.

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p-C3G Antibody (G-2) References:

1. Crk activation of JNK via C3G and R-Ras.  |  Mochizuki, N., et al. 2000. J Biol Chem. 275: 12667-71. PMID: 10777559
2. Requirement for C3G-dependent Rap1 activation for cell adhesion and embryogenesis.  |  Ohba, Y., et al. 2001. EMBO J. 20: 3333-41. PMID: 11432821
3. C3G, a guanine nucleotide exchange factor bound to adapter molecule c-Crk, has two alternative splicing forms.  |  Zhai, B., et al. 2001. Biochem Biophys Res Commun. 286: 61-6. PMID: 11485308
4. The guanine nucleotide exchange factor C3G is necessary for the formation of focal adhesions and vascular maturation.  |  Voss, AK., et al. 2003. Development. 130: 355-67. PMID: 12466202
5. Src-CrkII-C3G-dependent activation of Rap1 switches growth hormone-stimulated p44/42 MAP kinase and JNK/SAPK activities.  |  Ling, L., et al. 2003. J Biol Chem. 278: 27301-11. PMID: 12734187
6. Physical and functional interaction between Hck tyrosine kinase and guanine nucleotide exchange factor C3G results in apoptosis, which is independent of C3G catalytic domain.  |  Shivakrupa, R., et al. 2003. J Biol Chem. 278: 52188-94. PMID: 14551197
7. C3G-mediated suppression of oncogene-induced focus formation in fibroblasts involves inhibition of ERK activation, cyclin A expression and alterations of anchorage-independent growth.  |  Guerrero, C., et al. 2004. Oncogene. 23: 4885-93. PMID: 15077165
8. Amplification, up-regulation and over-expression of C3G (CRK SH3 domain-binding guanine nucleotide-releasing factor) in non-small cell lung cancers.  |  Hirata, T., et al. 2004. J Hum Genet. 49: 290-295. PMID: 15138850
9. Rap1 mutants with increased affinity for the guanine-nucleotide exchange factor C3G.  |  Shi, S., et al. 2004. Oncogene. 23: 8711-9. PMID: 15480424
10. Interaction of Bcr/Abl with C3G, an exchange factor for the small GTPase Rap1, through the adapter protein Crkl.  |  Cho, YJ., et al. 2005. Biochem Biophys Res Commun. 333: 1276-83. PMID: 15982636

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-C3G Antibody (G-2) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-p-C3G Antibody (G-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-C3G Antibody (G-2) Specifications

Alternate Product Name: p-RAPGEF1

Product Clone Name: G-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: Tyr 514

Epitope Target: C3G

Epitope Species: mouse

Product Reactivity/Detection: Tyr 514 phosphorylated C3G

Species Reactivity/Detection: mouse

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for p-C3G Antibody (G-2): P: Blocking Peptide

The preferred C3G antibody is C3G Antibody (G-4): sc-17840.