p-Src Antibody (H-8): sc-166859

p-c-Src Antibody (H-8) is a mouse monoclonal IgG2b antibody raised against human c-Src, also known as pp60Src, Src p60, and proto-oncogene tyrosine protein kinase Src, a crucial member of the Src gene family of membrane-associated tyrosine kinases. p-c-Src mouse monoclonal antibody (H-8) recognizes an epitope within c-Src and demonstrates broad species cross-reactivity, detecting c-Src in mouse, rat, and human samples through Western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry on paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA) applications. The Src gene family comprises nine members that maintain inactive states until specific cellular processes, such as mitosis, trigger their activation to regulate proliferation, differentiation, motility, and adhesion. c-Src′s structural organization includes an amino-terminal cell membrane anchor followed by SH3 and SH2 domains, which facilitate c-Src′s crucial role in signal transduction. c-Src shows particularly high expression in neuronal tissues and platelets, where c-Src contributes to neuronal differentiation and maintains mature neuronal cell functions. The ability of c-Src to distribute across different subcellular compartments proves essential for regulating specific cellular processes, including mitogenesis and cytoskeletal organization. Anti-p-c-Src monoclonal antibody (H-8) serves as an invaluable tool for researchers investigating these critical cellular pathways and regulatory mechanisms.

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p-Src Antibody (H-8) References:

1. Lyn kinase activity is the predominant cellular SRC kinase activity in glioblastoma tumor cells.  |  Stettner, MR., et al. 2005. Cancer Res. 65: 5535-43. PMID: 15994925
2. Molecular features of the viral and cellular Src kinases involved in interactions with the GTPase-activating protein.  |  Brott, BK., et al. 1991. Mol Cell Biol. 11: 5059-67. PMID: 1717825
3. Activation of the oncogenic potential of the avian cellular src protein by specific structural alteration of the carboxy terminus.  |  Reynolds, AB., et al. 1987. EMBO J. 6: 2359-64. PMID: 2822389
4. Enhancement of cellular src gene product associated tyrosyl kinase activity following polyoma virus infection and transformation.  |  Bolen, JB., et al. 1984. Cell. 38: 767-77. PMID: 6091900
5. Differential expression of the cellular src gene during vertebrate development.  |  Schartl, M. and Barnekow, A. 1984. Dev Biol. 105: 415-22. PMID: 6207061
6. Viral and cellular src genes contribute to the structure of recovered avian sarcoma virus transforming protein.  |  Karess, RE. and Hanafusa, H. 1981. Cell. 24: 155-64. PMID: 6263484
7. The differential expression of the cellular src-gene product pp60src and its phosphokinase activity in normal chicken cells and tissues.  |  Barnekow, A. and Bauer, H. 1984. Biochim Biophys Acta. 782: 94-102. PMID: 6326834
8. Regulation of the cellular src protein tyrosine kinase: interactions of the carboxyl terminal sequences residing between the kinase domain and tyrosine-527.  |  Cobb, BS. and Parsons, JT. 1993. Oncogene. 8: 2897-903. PMID: 7692368
9. Association of Hsp90 with cellular Src-family kinases in a cell-free system correlates with altered kinase structure and function.  |  Hartson, SD. and Matts, RL. 1994. Biochemistry. 33: 8912-20. PMID: 8043579
10. Mechanism of biological synergy between cellular Src and epidermal growth factor receptor.  |  Tice, DA., et al. 1999. Proc Natl Acad Sci U S A. 96: 1415-20. PMID: 9990038

The preferred Src antibody is Src Antibody (B-12): sc-8056.