OTUB2 CRISPR/Cas9 KO Plasmid (h): sc-413099
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- OTUB2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the OTUB2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
OTUB2 CRISPR/Cas9 KO Plasmid (h) | sc-413099 | 20 µg | $397.00 |
Overview
OTUB2 (OTU deubiquitinase, ubiquitin aldehyde binding 2) encodes a cysteine protease deubiquitinase that edits ubiquitin signals to regulate protein stability and signaling amplitude. By preferentially cleaving specific ubiquitin chain linkages, OTUB2 influences ubiquitin-dependent pathways controlling proteostasis, DNA damage responses, and stress-adaptive signaling cascades. Altered OTUB2 activity has been linked to dysregulated ubiquitin homeostasis observed across multiple disease contexts, including oncogenic signaling programs and inflammatory pathobiology. As a result, OTUB2 is frequently studied for its impact on pathway crosstalk between ubiquitination, transcriptional control, and cellular survival decisions.
OTUB2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the OTUB2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the OTUB2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the OTUB2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish OTUB2 protein expression.
This CRISPR knockout system enables efficient generation of OTUB2-deficient cell models for investigation of OTUB2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting OTUB2 exon(s) critical for OTUB2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple OTUB2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by OTUB2 CRISPR/Cas9 KO Plasmid (h) and OTUB2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the OTUB2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by OTUB2 HDR Plasmid (h) and OTUB2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by OTUB2 homology arms to support homology-directed repair at defined OTUB2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.