OSBP Antibody (H-5): sc-515683

OSBP Antibody (H-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects OSBP protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-OSBP antibody (H-5) recognizes Oxysterol-binding protein (OSBP), which plays a crucial role in lipid metabolism and signal transduction, as well as vesicle transport. OSBP (H-5) antibody targets a protein essential for transporting sterols from lysosomes to the nucleus, where OSBP regulates expression of genes involved in cholesterol synthesis, including HMG synthetase, HMG-CoA reductase, and low-density lipoprotein receptor (LDLR). OSBP is primarily localized in the cytosol and widely expressed across various tissues, while OSBP2 is predominantly found in the testis, retina, and fetal liver. OSBP (H-5) monoclonal antibody detects a protein that interacts with 25-hydroxy-cholesterol (25-HC), a potent modulator of cholesterol synthesis gene transcription, thereby influencing extracellular signal-regulated kinase (ERK) signaling pathway through cholesterol-binding properties. OSBP′s ability to translocate to Golgi membrane periphery upon binding to oxysterols highlights a dynamic role in cellular lipid homeostasis and signaling, making anti-OSBP antibody (H-5) an invaluable tool for studying these critical biological processes.

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OSBP Antibody (H-5) References:

1. The OSBP-related proteins (ORPs): global sterol sensors for co-ordination of cellular lipid metabolism, membrane trafficking and signalling processes?  |  Olkkonen, VM., et al. 2006. Biochem Soc Trans. 34: 389-91. PMID: 16709169
2. OSBP-related proteins: liganding by glycerophospholipids opens new insight into their function.  |  Olkkonen, VM. 2013. Molecules. 18: 13666-79. PMID: 24196413
3. OSBP-Related Protein Family in Lipid Transport Over Membrane Contact Sites.  |  Olkkonen, VM. 2015. Lipid Insights. 8: 1-9. PMID: 26715851
4. OSBP-Related Protein Family: Mediators of Lipid Transport and Signaling at Membrane Contact Sites.  |  Kentala, H., et al. 2016. Int Rev Cell Mol Biol. 321: 299-340. PMID: 26811291
5. OSBP-related protein 2 (ORP2): Unraveling its functions in cellular lipid/carbohydrate metabolism, signaling and F-actin regulation.  |  Olkkonen, VM., et al. 2019. J Steroid Biochem Mol Biol. 192: 105298. PMID: 30716465
6. Differing activities of oxysterol-binding protein (OSBP) targeting anti-viral compounds.  |  Roberts, BL., et al. 2019. Antiviral Res. 170: 104548. PMID: 31271764
7. Coordination of inter-organelle communication and lipid fluxes by OSBP-related proteins.  |  Arora, A., et al. 2022. Prog Lipid Res. 86: 101146. PMID: 34999137
8. A phosphoinositide signalling pathway mediates rapid lysosomal repair.  |  Tan, JX. and Finkel, T. 2022. Nature. 609: 815-821. PMID: 36071159
9. New insights into the OSBP‒VAP cycle.  |  Subra, M., et al. 2023. Curr Opin Cell Biol. 82: 102172. PMID: 37245352
10. OSBP-mediated PI(4)P-cholesterol exchange at endoplasmic reticulum-secretory granule contact sites controls insulin secretion.  |  Panagiotou, S., et al. 2024. Cell Rep. 43: 113992. PMID: 38536815

The preferred OSBP antibody is OSBP Antibody (A-5): sc-365771.