ORNT1 CRISPR/Cas9 KO Plasmid (m): sc-422074
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ORNT1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ORNT1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ORNT1 CRISPR/Cas9 KO Plasmid (m) | sc-422074 | 20 µg | $397.00 |
Overview
Slc25a15 encodes the mitochondrial ornithine transporter ORNT1, a member of the SLC25 carrier family that mediates ornithine exchange across the inner mitochondrial membrane. In mouse, ORNT1 supports the urea cycle by supplying mitochondrial ornithine for carbamoylation and facilitating citrulline/ornithine flux coupled to nitrogen disposal and amino acid catabolism. This transport activity links mitochondrial metabolism to cytosolic nitrogen handling, influencing arginine and polyamine-related metabolic networks. Dysregulation of ornithine transport and urea cycle flux is associated with hyperornithinemia-related metabolic phenotypes and can impact cellular responses to nitrogen stress.
ORNT1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Slc25a15 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Slc25a15 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Slc25a15 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ORNT1 protein expression.
This CRISPR knockout system enables efficient generation of Slc25a15-deficient cell models for investigation of ORNT1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Slc25a15 exon(s) critical for ORNT1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Slc25a15 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ORNT1 CRISPR/Cas9 KO Plasmid (m) and ORNT1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Slc25a15 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ORNT1 HDR Plasmid (m) and ORNT1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Slc25a15 homology arms to support homology-directed repair at defined Slc25a15 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.