OMP Double Nickase Plasmid (h): sc-403257-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- OMP Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- OMP Double Nickase Plasmid (h) and OMP Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting OMP. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: OMP Antibody (B-6): sc-365818
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
OMP Double Nickase Plasmid (h) | sc-403257-NIC | 20 µg | $410.00 |
Olfactory marker protein (OMP) is a small cytosolic protein highly enriched in mature olfactory sensory neurons, where it supports odorant signal transduction and neuronal maturation. OMP participates in processes linked to cAMP-dependent signaling, calcium handling, and adaptation kinetics downstream of odorant receptor activation, shaping the timing and fidelity of sensory responses. Altered OMP expression is widely used as a molecular indicator of olfactory neuron differentiation and epithelial remodeling in studies of anosmia and other olfactory dysfunction phenotypes, including those associated with inflammation or neurodegenerative contexts. As a lineage and functional marker, OMP is frequently examined in models of neuronal development, regeneration, and sensory circuit maintenance.
OMP Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the OMP locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within OMP. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt OMP function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of OMP-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.