Olfr988 Double Nickase Plasmid (m): sc-434380-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Olfr988 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Olfr988 Double Nickase Plasmid (m) and Olfr988 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Olfr988. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Olfr988 Double Nickase Plasmid (m) | sc-434380-NIC | 20 µg | $410.00 | |||
Olfr988 Double Nickase Plasmid (m2) | sc-434380-NIC-2 | 20 µg | $410.00 |
Olfr988 encodes an odorant receptor in the mouse olfactory receptor (OR) family, a class A GPCR primarily involved in chemosensory detection and signal transduction in olfactory sensory neurons. Upon ligand binding, ORs couple to G protein–dependent pathways that elevate cAMP and drive downstream ion channel activation, supporting neuronal depolarization and odor-evoked responses. Beyond canonical olfaction, ectopic OR expression has been reported in select peripheral tissues, prompting investigation into potential roles in cell signaling and tissue-specific gene regulation. Dysregulated GPCR signaling and altered sensory receptor repertoires are relevant to studies of olfactory dysfunction and broader GPCR-mediated physiological processes.
Olfr988 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Olfr988 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Olfr988. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Olfr988 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Olfr988-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.