ODCp Double Nickase Plasmid (h): sc-410634-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ODCp Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ODCp Double Nickase Plasmid (h) and ODCp Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting AZIN2. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ODCp Double Nickase Plasmid (h) | sc-410634-NIC | 20 µg | $410.00 | |||
ODCp Double Nickase Plasmid (h2) | sc-410634-NIC-2 | 20 µg | $410.00 |
Human AZIN2 encodes ornithine decarboxylase paralog (ODCp), a polyamine-pathway regulator that modulates cellular levels of putrescine, spermidine, and spermine by interacting with antizyme-dependent control of decarboxylase activity and turnover. Through this regulation, AZIN2 contributes to polyamine homeostasis, influencing fundamental processes such as translation, cell-cycle progression, vesicular trafficking, and differentiation. Dysregulated polyamine metabolism is frequently linked to altered proliferation and stress responses, making AZIN2/ODCp relevant for studying metabolic rewiring and growth control across disease-associated cellular states. AZIN2 expression has also been associated with specialized secretory phenotypes in select tissues, supporting investigation of polyamine-dependent regulation of secretion and cellular maturation.
ODCp Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the AZIN2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within AZIN2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt AZIN2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of AZIN2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.