Nucleoredoxin Double Nickase Plasmid (h): sc-413004-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Nucleoredoxin Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Nucleoredoxin Double Nickase Plasmid (h) and Nucleoredoxin Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting NXN. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Nucleoredoxin Antibody (E-12): sc-393748
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Nucleoredoxin Double Nickase Plasmid (h) | sc-413004-NIC | 20 µg | $410.00 |
Human NXN encodes nucleoredoxin, a thioredoxin family oxidoreductase that couples cellular redox status to signaling outputs by catalyzing reversible cysteine redox modifications on protein targets. Nucleoredoxin has been linked to regulation of Wnt/β-catenin signaling through redox-dependent control of Dishevelled interactions, thereby influencing transcriptional programs involved in proliferation, differentiation, and tissue homeostasis. It also contributes to oxidative stress responses and redox-sensitive control of protein function within the cytosol and associated complexes. Dysregulated NXN activity and altered redox signaling have been investigated in the context of inflammation, neurobiology, metabolic stress, and cancer-related pathway rewiring, making it a useful node for mechanistic studies of redox-regulated signaling networks.
Nucleoredoxin Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the NXN locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within NXN. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt NXN function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of NXN-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.