NSUN6 Double Nickase Plasmid (h): sc-415118-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NSUN6 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- NSUN6 Double Nickase Plasmid (h) and NSUN6 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting NSUN6. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: NSUN6 Antibody (D-5): sc-393446
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NSUN6 Double Nickase Plasmid (h) | sc-415118-NIC | 20 µg | $410.00 |
NSUN6 encodes an RNA cytosine-5 methyltransferase that installs m5C modifications on specific RNA substrates, contributing to epitranscriptomic control of RNA stability, processing, and translation. Through regulation of RNA modification patterns, NSUN6 can influence ribosome-associated gene expression programs and broader post-transcriptional networks that shape cellular growth and stress responses. Altered RNA methylation landscapes involving NSUN family enzymes have been linked to dysregulated differentiation and oncogenic signaling, making NSUN6 a useful node for studying how m5C marks impact phenotype. Research on NSUN6 supports mechanistic investigations into RNA modification–driven pathways in cancer biology and other conditions where RNA metabolism is perturbed.
NSUN6 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the NSUN6 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within NSUN6. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt NSUN6 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of NSUN6-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.